The nephroprotective effect of amifostine in a cecal ligation-induced sepsis model in terms of oxidative stress and inflammation

-OBJECTIVE: Sepsis is responsi-ble for more than 5 million deaths worldwide ev-ery year. The purpose of this study was to use amifostine to reduce acute kidney injury devel-oping as a result of sepsis.MATERIALS AND METHODS: Thirty Sprague Dawley rats were divided into three equal groups - a healthy control group (Group 1), cecal liga-tion and puncture group (CLP, Group 2), and a CLP + amifostine (AMF) group receiving a total of 200 mg/kg AMF intraperitoneally (i.p.) 15 min before sepsis induction (Group 3).RESULTS: Total thiol levels decreased while malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappa B (NF-Kappa B/p65), and interleukin (IL)-1 beta, and IL-6 levels increased in the CLP group. We also observed degeneration in re -nal corpuscles, necrotic tubules, polymorphonu-clear leukocyte inflammation, and vascular con- gestion. In the amifostine group, total thiol levels in tissue increased, while MDA, TNF-alpha, NF-kB/ p65, IL-1 beta, and IL-6 levels, necrotic renal tubules, and inflammation decreased.CONCLUSIONS: Amifostine prevented sep-sis-related acute kidney injury by reducing in-flammation and oxidative stress.