A Comprehensive Analysis of the DUF4228 Gene Family in Gossypium Reveals the Role of GhDUF4228-67 in Salt Tolerance

被引：4
作者：
Lv, Xiaoyan [1 ]
Wei, Fei [1 ,2 ]
Lian, Boying [1 ]
Yin, Guo [3 ]
Sun, Mengxi [1 ]
Chen, Pengyun [1 ]
An, Li [1 ]
Jian, Hongliang [1 ]
Wang, Hantao [1 ]
Fu, Xiaokang [1 ]
Ma, Liang [1 ]
Lu, Jianhua [1 ]
Wang, Baoquan [1 ]
Wei, Hengling [1 ]
机构：
[1] Chinese Acad Agr Sci, State Key Lab Cotton Biol, Inst Cotton Res, Anyang 455000, Peoples R China
[2] Zhengzhou Univ, Zhengzhou Univ Res Base, Sch Agr Sci, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China
[3] Handan Acad Agr Sci, Handan 056000, Peoples R China
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 21期
关键词：
Gossypium; domains of unknown function 4228 (DUF4228); VIGS; salt stress; EXPRESSION ANALYSIS; DROUGHT STRESS; PROTEIN; RESISTANCE; PROLINE; PLANTS; TOOLS;
D O I：
10.3390/ijms232113542
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Soil salinization conditions seriously restrict cotton yield and quality. Related studies have shown that the DUF4228 proteins are pivotal in plant resistance to abiotic stress. However, there has been no systematic identification and analysis of the DUF4228 gene family in cotton and their role in abiotic stress. In this study, a total of 308 DUF4228 genes were identified in four Gossypium species, which were divided into five subfamilies. Gene structure and protein motifs analysis showed that the GhDUF4228 proteins were conserved in each subfamily. In addition, whole genome duplication (WGD) events and allopolyploidization might play an essential role in the expansion of the DUF4228 genes. Besides, many stress-responsive (MYB, MYC) and hormone-responsive (ABA, MeJA) related cis-elements were detected in the promoters of the DUF4228 genes. The qRT-PCR results showed that GhDUF4228 genes might be involved in the response to abiotic stress. VIGS assays and the measurement of relative water content (RWC), Proline content, POD activity, and malondialdehyde (MDA) content indicated that GhDUF4228-67 might be a positive regulator of cotton response to salt stress. The results in this study systematically characterized the DUF4228s in Gossypium species and will provide helpful information to further research the role of DUF4228s in salt tolerance.
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页数：20
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