Expression at a 20L scale and purification of the extracellular domain of the Schistosoma mansoni TSP-2 recombinant protein A vaccine candidate for human intestinal schistosomiasis

被引：27

作者：

Curti, Elena ^{[1
,2
,3
,4
]}

Kwityn, Clifford ^{[1
,2
,3
,4
]}

Zhan, Bin ^{[1
,2
,3
,4
]}

Gillespie, Portia ^{[1
,2
,3
,4
]}

Brelsford, Jill ^{[5
]}

Deumic, Vehid ^{[5
]}

Plieskatt, Jordan ^{[5
]}

Rezende, Wanderson C. ^{[1
,2
,3
,4
]}

Tsao, Eric ^{[6
]}

Kalampanayil, Bose ^{[6
]}

Hotez, Peter J. ^{[1
,2
,3
,4
,7
]}

Bottazzi, Maria Elena ^{[1
,2
,3
,4
]}

机构：

[1] Baylor Coll Med, Dept Pediat, Natl Sch Trop Med, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Mol Virol & Microbiol, Natl Sch Trop Med, Houston, TX 77030 USA

[3] Sabin Vaccine Inst, Houston, TX USA

[4] Texas Childrens Hosp, Ctr Vaccine Dev, Houston, TX 77030 USA

[5] George Washington Univ, Dept Microbiol Immunol & Trop Med, Washington, DC USA

[6] Aeras, Rockville, MD USA

[7] Rice Univ, James A Baker III Inst Publ Policy, Houston, TX USA

来源：

HUMAN VACCINES & IMMUNOTHERAPEUTICS | 2013年 / 9卷 / 11期

关键词：

Schistosomiasis; Schistosoma mansoni; neglected tropical diseases; vaccine; Sm-TSP-2; Pichia Pastoris; recombinant protein; SUB-SAHARAN AFRICA; PROTEOMIC ANALYSIS; SURFACE-MEMBRANES; PRAZIQUANTEL; INFECTION; ANTIGENS; TEGUMENT;

D O I：

10.4161/hv.25787

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel recombinant protein vaccine for human schistosomiasis caused by Schistosoma mansoni is under development. The Sm-TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. Here, we describe the cloning and the expression of the external loop of Sm-TSP-2 recombinant protein secreted by Pichia Pink (TM) the process development at 20L scale fermentation, and the two-steps purification, which resulted in a protein recovery yield of 31% and a protein purity of 97%. The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies.

引用

页码：2342 / 2350

页数：9

共 1 条

[1] Biophysical and formulation studies of the Schistosoma mansoni TSP-2 extracellular domain recombinant protein, a lead vaccine candidate antigen for intestinal schistosomiasis
Cheng, Weiqiang
Curti, Elena
Rezende, Wanderson C.
Kwityn, Clifford
Zhan, Bin
Gillespie, Portia
Plieskatt, Jordan
Joshi, Sangeeta B.
Volkin, David B.
Hotez, Peter J.
Middaugh, C. Russell
Bottazzi, Maria Elena
HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2013, 9 (11) : 2351 - 2361

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