The Role of Calcium-Dependent Protein Kinase Genes CPK16, CPK25, CPK30, and CPK32 in Stilbene Biosynthesis and the Stress Resistance of Grapevine Vitis amurensis Rupr.

被引：18
作者：
Dubrovina, A. S. [1 ]
Aleynova, O. A. [1 ]
Manyakhin, A. Y. [1 ]
Kiselev, K. V. [1 ]
机构：
[1] Russian Acad Sci, Far Eastern Branch, Fed Sci Ctr Biodivers Terr Biota East Asia, Vladivostok 690022, Russia
来源：
APPLIED BIOCHEMISTRY AND MICROBIOLOGY | 2018年 / 54卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
calcium-dependent protein kinases; abiotic stresses; stilbene biosynthesis; Vitis amurensis; TRANSGENIC CELL-CULTURES; RESVERATROL PRODUCTION; EXPRESSION; PLANTS; ARABIDOPSIS; FAMILY;
D O I：
10.1134/S0003683818040051
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
It is known that calcium-dependent protein kinases (CDPK or CPK) are implicated in the regulation of plant development and stress adaptation. However, there is a lack of information on the properties and functions of certain CDPK family members. The present study investigates the functions of four CDPK genes of the grapevine Vitis amurensis Rupr. in the formation of its high stress resistance level and the production of valuable secondary metabolites. Overexpression of the CPK30 gene of V. amurensis considerably increased the resistance of V. amurensis transgenic cell lines to salt and cold stresses, while CPK16, CPK25, and CPK32 overexpression did not influence the salt and temperature stress tolerance. VaCPK16 and VaCPK32 overexpression increased stilbene production in V. amurensis cell cultures by 2.1-3.1 and 1.6-3.1 times, respectively. The data indicate that the VaCPK30 gene is involved in the formation of grapevine salt and cold stress resistance, while the VaCPK16 and VaCPK32 genes contribute to increased stilbene accumulation.
引用
收藏
页码：410 / 417
页数：8
相关论文
共 32 条
[1]   Activation of stilbene synthesis in cell cultures of Vitis amurensis by calcium-dependent protein kinases VaCPK1 and VaCPK26 [J].
Aleynova, O. A. ;
Dubrovina, A. S. ;
Kiselev, K. V. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2017, 130 (01) :141-152
[2]   Stilbene accumulation in cell cultures of Vitis amurensis Rupr. overexpressing VaSTS1, VaSTS2, and VaSTS7 genes [J].
Aleynova, O. A. ;
Grigorchuk, V. P. ;
Dubrovina, A. S. ;
Rybin, V. G. ;
Kiselev, K. V. .
PLANT CELL TISSUE AND ORGAN CULTURE, 2016, 125 (02) :329-339
[3]   Regulation of Resveratrol Production in Vitis amurensis Cell Cultures by Calcium-Dependent Protein Kinases [J].
Aleynova, O. A. ;
Dubrovina, A. S. ;
Manyakhin, A. Y. ;
Karetin, Y. A. ;
Kiselev, K. V. .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 175 (03) :1460-1476
[4]   VaCPK20 gene overexpression significantly increased resveratrol content and expression of stilbene synthase genes in cell cultures of Vitis amurensis Rupr [J].
Aleynova-Shumakova, O. A. ;
Dubrovina, A. S. ;
Manyakhin, A. Y. ;
Karetin, Y. A. ;
Kiselev, K. V. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 98 (12) :5541-5549
[5]   Analysis of calcium signaling pathways in plants [J].
Batistic, Oliver ;
Kudla, Joerg .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2012, 1820 (08) :1283-1293
[6]   CDPKs in immune and stress signaling [J].
Boudsocq, Marie ;
Sheen, Jen .
TRENDS IN PLANT SCIENCE, 2013, 18 (01) :30-40
[7]   Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family [J].
Cheng, SH ;
Willmann, MR ;
Chen, HC ;
Sheen, J .
PLANT PHYSIOLOGY, 2002, 129 (02) :469-485
[8]   Expressional Analysis and Role of Calcium Regulated Kinases in Abiotic Stress Signaling [J].
Das, Ritika ;
Pandey, Girdhar K. .
CURRENT GENOMICS, 2010, 11 (01) :2-13
[9]   Regulation of stilbene biosynthesis in plants [J].
Dubrovina, A. S. ;
Kiselev, K. V. .
PLANTA, 2017, 246 (04) :597-623
[10]   The calcium-dependent protein kinase gene VaCPK29 is involved in grapevine responses to heat and osmotic stresses [J].
Dubrovina, Alexandra S. ;
Kiselev, Konstantin V. ;
Khristenko, Valeriya S. ;
Aleynova, Olga A. .
PLANT GROWTH REGULATION, 2017, 82 (01) :79-89
1234