Ectopic expression of sorbitol-6-phosphate 2-dehydrogenase gene from Haloarcula marismortui enhances salt tolerance in transgenic Arabidopsis thaliana

被引：3
作者：
Gao, Jian-jie [1 ,4 ]
Sun, Yu-ru [1 ,2 ]
Zhu, Bo [1 ,4 ]
Peng, Ri-He [1 ,4 ]
Wang, Bo [1 ,4 ]
Wang, Li-Juan [1 ,4 ]
Li, Zhen-Jun [1 ,4 ]
Chen, Lei [3 ]
Yao, Quan-Hong [1 ,4 ]
机构：
[1] Shanghai Acad Agr Sci, Biotechnol Res Inst, 2901 Beidi Rd, Shanghai 201106, Peoples R China
[2] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China
[3] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China
[4] Shanghai Acad Agr Sci, Shanghai Key Lab Agr Genet & Breeding, Shanghai 201106, Peoples R China
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 2018年 / 40卷 / 06期
关键词：
Sorbitol-6-phosphate; 2-dehydrogenase; Transgenic Arabidopsis; Salt stress; Haloarcula marismortui; SORBITOL DEHYDROGENASE; SUGAR METABOLISM; STRESS; APPLE; CDNA; TRANSFORMATION; SUCROSE; PURIFICATION; GERMINATION; MODULATION;
D O I：
10.1007/s11738-018-2668-x
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
D-Sorbitol-6-phosphate 2-dehydrogenase (S6PDH, E.C. 1.1.1.140) catalyzes the NADH-dependent conversion of D-fructose 6-phosphate (F6P) to D-sorbitol 6-phosphate (S6P). In this work, recombination and characterization of Haloarcula marismortui D-sorbitol-6-phosphate 2-dehydrogenase are reported. Haloarcula marismortui D-sorbitol-6-phosphate 2-dehydrogenase was expressed in P. pastoris and Arabidopsis thaliana. Enzyme assay indicated that HmS6PDH catalyzes the reduction of D-fructose 6-phosphate to D-sorbitol 6-phosphate and HmS6PDH activity was enhanced by NaCl. Furthermore, transgenic A. thaliana ectopic expressing HmS6PDH accumulate more sorbitol under salt stress. These results suggest that the ectopic expression of HmS6PDH in plants can facilitate future studies regarding the engineering and breeding of salt-tolerant crops.
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页数：11
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