Single-cell transcriptional diversity of neonatal umbilical cord blood immune cells reveals neonatal immune tolerance

被引:6
作者
Shi, Xuyang [1 ,2 ]
Ma, Wen [2 ]
Duan, Shanshan [2 ]
Shi, Quan [2 ]
Wu, Shuo [2 ,3 ]
Hao, Shijie [1 ,2 ]
Dong, Guoyi [2 ]
Li, Jinxiu [1 ,2 ]
Song, Yumo [2 ]
Liu, Chang [2 ]
Lin, Xiumei [1 ,2 ]
Yuan, Yue [2 ]
Deng, Qiuting [1 ,2 ]
Xu, Jiangshan [1 ,2 ]
Bai, Shiyi [2 ]
Hou, Yong [1 ,2 ,4 ]
Liu, Chuanyu [2 ]
Liu, Longqi [1 ,2 ]
机构
[1] Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
[2] BGI Shenzhen, Shenzhen 518083, Peoples R China
[3] Shenzhen Technol Univ, Coll Hlth Sci & Environm Engn, Shenzhen 518118, Peoples R China
[4] BGI Shenzhen, Shenzhen Key Lab Single Cell Omics, Shenzhen 518120, Peoples R China
基金
中国国家自然科学基金;
关键词
Neonatal immune tolerance; Umbilical cord blood; Differential gene expression; Single-cell RNA sequencing; Single-cell sequencing assay for; transposase-accessible chromatin; T-CELLS; RNA-SEQ; RESPONSES;
D O I
10.1016/j.bbrc.2022.03.132
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The characteristics of neonatal immune cells display intrinsic differences compared with adult immune cells. Therefore, a comprehensive analysis of key gene expression regulation is required to understand the response of the human fetal immune system to infections. Here, we applied single-cell RNA sequencing (scRNA-seq) and single-cell sequencing assay for transposase-accessible chromatin (scATACseq) to systematically profile umbilical cord blood (UCB) nucleated cells and peripheral blood mononuclear cells (PBMCs) to identify their composition and differentially expressed genes. The immune cells in neonatal UCB demonstrated the expression of key genes, such as HBG2, NFKBIA, JUN, FOS, and TNFAIP3. In contrast, natural killer and T cells, which are constituents of adult PBMCs, exhibited high cytotoxic gene expression. Furthermore, we obtained similar results from the data of scATAC-seq by identifying the status of chromatin accessibility of key genes. Therefore, scRNA-seq and scATAC-seq of neonatal UCB nucleated cells and adult PBMCs could serve as an invaluable resource for elucidating the regulatory mechanisms of responses of distinct immune cell types and further identifying the differences between neonatal and adult immune responses to predict the potential underlying mechanism for neonatal immune tolerance.(c) 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:14 / 22
页数:9
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