Characterization of fibrillar collagen isoforms in infarcted mouse hearts using second harmonic generation imaging

被引：7

作者：

Sahu, Sushant P. ^{[1
]}

Liu, Qianglin ^{[2
]}

Prasad, Alisha ^{[3
]}

Hasan, Sped Mohammad Abid ^{[3
]}

Liu, Qun ^{[4
]}

Rodriguez, Maria Ximena Bastidas ^{[4
]}

Mukhopadhyay, Orna ^{[5
]}

Burk, David ^{[6
]}

Francis, Joseph ^{[7
]}

Mukhopadhyay, Supratik ^{[4
]}

Fu, Xing ^{[2
]}

Gartia, Manas Ranjan ^{[3
]}

机构：

[1] Univ Louisiana Lafayette, Dept Chem, Lafayette, LA 70504 USA

[2] Louisiana State Univ, LSU AgCtr, Sch Anim Sci, Baton Rouge, LA 70803 USA

[3] Louisiana State Univ, Dept Mech & Ind Engn, Baton Rouge, LA 70803 USA

[4] Louisiana State Univ, Dept Comp Sci, Baton Rouge, LA 70803 USA

[5] Baton Rouge Magnet High Sch, Baton Rouge, LA 70806 USA

[6] Shared Instrumentat Facil & Pennington Biomed Res, Baton Rouge, LA 70808 USA

[7] Louisiana State Univ, Sch Vet Med, Comparat Biomed Sci, Baton Rouge, LA 70803 USA

来源：

BIOMEDICAL OPTICS EXPRESS | 2021年 / 12卷 / 01期

基金：

美国国家卫生研究院;

关键词：

ACUTE MYOCARDIAL-INFARCTION; ENDOMYOCARDIAL BIOPSY; IN-VIVO; CARDIOVASCULAR-DISEASE; CONNECTIVE-TISSUE; CARDIAC FIBER; SIRIUS RED; MICROSCOPY; FLUORESCENCE; QUANTIFICATION;

D O I：

10.1364/BOE.410347

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and immunofluorescence imaging using fluorescently-labeled collagen antibodies. The post-mortem cardiac tissue imaging using SHG demonstrated a progressive increase in collagen deposition in the left ventricle (LV) post-MI. We were able to monitor structural morphology and LV remodeling parameters in terms of extent of LV dilation, stiffness and fiber dimensions in the infarcted myocardium. (c) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

引用

页码：604 / 618

页数：15

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