Evaluation of DNA Oxidative Damage in Visceral Cells Using 8-OHdG level

we developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as testing material. The liver cells of rat were exposed to different concentrations of FA (0, 5, 15, 45 mu mol center dot L-1) for 1h, then the contents of 8-OHdG and MDA were measured. Research results showed that the 8-OHdG and MDA were increased gradually with the increase of formaldehyde concentration. At high FA concentration level (45 mu mol center dot L-1) the 8-OHdG and MDA contents are extremely different (P < 0.01) when compared with the control group, at the middle FA level (15 mu mol center dot L-1) their differences are significant (P < 0.01, P < 0.05), and at the low level (5 mu mol center dot L-1) there is no significant difference (P > 0.05). The experimental results also showed that the dose-effect relationship between FA and 8OHdG seems much better than that between FA and MDA. The conclusion is that 8-OHdG not only can be used for blood and urine sample testing, but also a good biomarker of visceral cell DNA oxidative damage, as it was explored in this study.