Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures

被引：9

作者：

Hong, Sung Kuk ^{[1
]}

Chang, Beung Ki ^{[1
]}

Song, Sang Hoon ^{[1
]}

Kim, Eui-Chong ^{[1
]}

机构：

[1] Seoul Natl Univ Hosp, Dept Lab Med, Seoul, South Korea

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2014年 / 32卷 / 04期

关键词：

Blood culture; MALDI-TOF MS; rapid bacterial identification; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; MICROBIOLOGY; BOTTLES;

D O I：

10.4103/0255-0857.142261

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We evaluated the feasibility of same-day routine aerobic bacterial identification using the following procedures: Picking colonies from 4 and 6 h incubated subculture from positive blood culture bottle and analyzing them by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The matched identification rate of this procedure at the species level was 80.6% (141/175) for the 4-h cultures compared with overnight cultures and 90.9% (159/175) for the 6-h cultures. Thus, our technique provides an easy and rapid method for identification of aerobic bacteria in routine clinical microbiology laboratories.

引用

页码：419 / 422

页数：4

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