Electrochemical proteolytic beacon for detection of matrix metalloproteinase activities

被引：76

作者：

Liu, Guodong ^{[1
]}

Wang, Jun ^{[1
]}

Wunschel, David S. ^{[1
]}

Lin, Yuehe ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Richland, WA 99352 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 38期

关键词：

D O I：

10.1021/ja0626638

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This communication describes a novel method for detecting matrix metalloproteinase-7 activity using a peptide substrate labeled with a ferrocene reporter. The substrate serves as a selective "electrochemical proteolytic beacon" (EPB) for this metalloproteinase. The EPB is immobilized on a gold electrode surface to enable "on-off" electrochemical signaling capability for uncleaved and cleaved events. The EPB is efficiently and selectively cleaved by MMP-7 as measured by the rate of decrease in redox current of ferrocene. Direct transduction of a signal corresponding to peptide cleavage events into an electronic signal thus provides a simple, sensitive route for detecting the MMP activity. The new method allows for identification of the activity of MMP-7 in concentrations as low as 3.4 pM. The concept can be extended to design a multiple peptide substrate labeled with different electroactive reporters for assaying multiple MMPs activities. Copyright © 2006 American Chemical Society.

引用

页码：12382 / 12383

页数：2

共 16 条

[1] MATRIX METALLOPROTEINASES - A REVIEW [J].