Aiming to evaluate the production and physiological quality of soybean seeds produced in different lines crushed by the tractor wheels during applications of pesticides, an experiment was conducted in the municipality of Jari - RS, during the 2008/2009 harvest. It was determined the height of the plants still in the field and were later collected five lines located in different positions relative to the wheeled tractor. After harvesting, to identify possible reductions in productivity and quality changes in physiological, seeds were brought to the Didactic Laboratory Seed Analysis at the Faculty of Agronomy Eliseu Maciel, Federal University of Pelotas. The seed yield were evaluated and performed tests of germination, first count, accelerated aging, tetrazolium, weight of 100 seeds, green seeds and hypochlorite test. In conditions in which the work was performed, kneading lines rotated by tractor reduces by 50% the production lines wrinkled in both cultivars. The percentage of normal seedlings, evaluated through quality tests, decreases in wrinkled lines, being more evident in the CD 219 RR. The CD 219 RR is more sensitive to the crumpling of the lines in relation to cultivate the RR 6001, when analyzed by the tetrazolium test.