Development of an ultrasensitive ic-ELISA and immunochromatographic strip assay for the simultaneous detection of florfenicol and thiamphenicol in eggs

被引：40
作者：
Lei, Xianlu [1 ,2 ]
Xu, Liguang [1 ,2 ]
Song, Shanshan [1 ,2 ]
Liu, Liqiang [1 ,2 ]
Kuang, Hua [1 ,2 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi, Jiangsu, Peoples R China
来源：
FOOD AND AGRICULTURAL IMMUNOLOGY | 2018年 / 29卷 / 01期
关键词：
Florfenicol; ic-ELISA; immunochromatographic strip assay; monoclonal antibody; thiamphenicol; ENZYME-LINKED-IMMUNOSORBENT; MONOCLONAL-ANTIBODY; LIQUID-CHROMATOGRAPHY; RAPID DETECTION; DRINKING-WATER; MILK SAMPLES; CHLORAMPHENICOL; AMINE; RACTOPAMINE; FEED;
D O I：
10.1080/09540105.2017.1371114
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
An ultrasensitive monoclonal antibody-based gold nanoparticle immunochromatographic strip assay and indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) were developed to detect florfenicol (FF) and thiamphenicol (TAP) in egg samples?. ? ? The ic-ELISA, with optimized pH, methanol content and sodium chloride content, exhibited an IC50 value of 0.2ng/mL for FF and 0.27ng/mL for TAP, with the working range of 0.05-0.77 and 0.05-1.42ng/mL, respectively. The optimized ic-ELISA showed negligible cross-reactivity with other phenols and broad-spectrum antibiotics. The recoveries in egg samples using the ic-ELISA ranged from 84% to 115% with a coefficient of variation of less than 5%. Based on this monoclonal antibody, a rapid and ultrasensitive immunochromatographic strip assay was developed with a cutoff value of 1ng/mL for FF and TAP. Our results indicated that both developed methods were highly useful for screening FF and TAP in eggs.
引用
收藏
页码：254 / 266
页数：13
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