Fast two-dimensional standing-wave total-internal-reflection fluorescence microscopy using acousto-optic deflectors

被引：16

作者：

Gliko, Olga ^{[2
]}

Brownell, William E. ^{[1
,2
]}

Saggau, Peter ^{[1
]}

机构：

[1] Baylor Coll Med, Dept Neurosci, Houston, TX 77030 USA

[2] Baylor Coll Med, Dept Otorhinolaryngol Head & Neck Surg, Houston, TX 77030 USA

来源：

OPTICS LETTERS | 2009年 / 34卷 / 06期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

STRUCTURED-ILLUMINATION MICROSCOPY; RESOLUTION; LIMIT;

D O I：

10.1364/OL.34.000836

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report a scheme for 2D standing-wave total-internal-reflection fluorescence microscopy. Standing-wave patterns are generated by two interfering beams coupled through the objective lens. Period, angular orientation, and phase of standing waves are controlled entirely by acousto-optic deflectors. The lateral resolution improvement of 100 nm is combined with an axial selectivity of <100 nm. by utilizing an evanescent standing-wave pattern. This technique can provide real-time imaging of subresolution structures in live biological specimens near a glass-water interface. (C) 2009 Optical Society of America

引用

页码：836 / 838

页数：3

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