A bicistronic subgenornic mRNA encodes both the ORF2 and ORF3 proteins of hepatitis E virus

被引：217

作者：

Graff, Judith ^{[1
]}

Torian, Udana ^{[1
]}

Nguyen, Hanh ^{[1
]}

Emerson, Suzanne U. ^{[1
]}

机构：

[1] NIH, NIAID, LID, Mol Hepatitis Sect, Bethesda, MD 20892 USA

来源：

JOURNAL OF VIROLOGY | 2006年 / 80卷 / 12期

关键词：

D O I：

10.1128/JVI.00046-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Hepatitis E virus replicons containing the neomycin resistance gene expressed from open reading frames (ORFs) 2 and 3 were transfected into Huh-7 cells, and stable cell lines containing functional replicons were selected by constant exposure to G418 sulfate. Northern blot analyses detected full-length replicon RNA and a single subgenomic RNA. This subgenomic RNA, which was capped, initiated at nucleotide 5122 downstream of the first two methionine codons in ORF3 and was bicistronic; two closely spaced methionine codons in different reading frames were used for the initiation of ORF3 and ORF2 translation.

引用

页码：5919 / 5926

页数：8

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