rAAV/ABAD-DP-6His attenuates oxidative stress-induced injury of PC12 cells

被引：2
|
作者：
Jia, Mingyue [1 ]
Wang, Mingyu [2 ]
Yang, Yi [1 ]
Chen, Yixin [3 ]
Liu, Dujuan [4 ]
Wang, Xu [1 ]
Song, Lei [1 ]
Wu, Jiang [1 ]
Yang, Yu [1 ]
机构：
[1] Jilin Univ, Hosp 1, Dept Neurol, Changchun 130021, Jilin Province, Peoples R China
[2] Peoples Hosp Jilin Prov, Dept Neurol, Changchun, Jilin Province, Peoples R China
[3] Jilin Univ, Coll Publ Hlth, Changchun 130021, Jilin Province, Peoples R China
[4] CNPC Jilin, Gen Hosp, Dept Burn & Plast Surg, Changchun, Jilin Province, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2014年 / 9卷 / 05期
基金：
中国国家自然科学基金;
关键词：
nerve regeneration; neurodegenerative disease; gene therapy; Alzheimer's disease; amyloid beta peptide; amyloid beta binding alcohol dehydrogenase; adeno-associated virus; hydrogen peroxide; oxidative stress; mitochondrial dysfunction; NSFC grant; neural regeneration; AMYLOID-BETA PEPTIDE; A-BETA; ALZHEIMERS-DISEASE; CALCIUM-TRANSPORT; FREE-RADICALS; MOUSE MODEL; IN-VIVO; SUPEROXIDE; DAMAGE; PROTEIN;
D O I：
10.4103/1673-5374.130065
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Our previous studies have revealed that amyloid beta (A beta)-binding alcohol dehydrogenase (ABAD) decoy peptide antagonizes A beta(42)-induced neurotoxicity. However, whether it improves oxidative stress injury remains unclear. In this study, a recombinant adenovirus constitutively secreting and expressing A beta-ABAD decoy peptide (rAAV/ABAD-DP-6His) was successfully constructed. Our results showed that rAAV/ABAD-DP-6His increased superoxide dismutase activity in hydrogen peroxide-induced oxidative stress-mediated injury of PC12 cells. Moreover, rAAV/ABADDP-6His decreased malondialdehyde content, intracellular Ca2+ concentration, and the level of reactive oxygen species. rAAV/ABAD-DP-6His maintained the stability of the mitochondrial membrane potential. In addition, the ATP level remained constant, and apoptosis was reduced. Overall, the results indicate that rAAV/ABAD-DP-6His generates the fusion peptide, A beta-ABAD decoy peptide, which effectively protects PC12 cells from oxidative stress injury induced by hydrogen peroxide, thus exerting neuroprotective effects.
引用
收藏
页码：481 / 488
页数：8
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