Quantification of ganciclovir in human plasma using capillary electrophoresis

被引:11
作者
Saleh, Soundos
Hempel, Georg
机构
[1] Univ Munster, Inst Pharmazeut & Med Chem, D-48149 Munster, Germany
[2] Klin & Poliklin Kinder & Jugendmed Padiatr Hamato, Munster, Germany
关键词
acyclovir; bubble cell; ganciclovir; plasma; ultrafiltration;
D O I
10.1002/elps.200500903
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A fast, simple, specific capillary electrophoretic method in the MEKC mode for the quantification of the antiviral drug ganciclovir is described. The separation was obtained using a 50 mu m id fused-silica capillary, 60 mM borax buffer (pH 9.25) containing 40 mM SDS using ethenoadenosine as the internal standard. Sample preparation was done by ultrafiltration with a Microcon 30000 kDa filter. The analytes were detected with UV detector at 254 nm. A sufficient sensitivity was achieved by using a bubble cell capillary. The linear range was from 0.5 to 10 mg/L with a LOQ of 0.5 mg/L. Correlation coefficients were better than 0.999 whereas inter- and intraday precision and accuracy were less than 10.7%. The analysis of patients' samples after administration of ganciclovir indicates that the method is suitable for drug monitoring in the clinic.
引用
收藏
页码:2439 / 2443
页数:5
相关论文
共 50 条
  • [21] Electrically Assisted Liquid- Phase Microextraction Combined With Capillary Electrophoresis for Quantification of Propranolol Enantiomers in Human Body Fluids
    Tabani, Hadi
    Fakhari, Ali Reza
    Shahsavani, Abolfath
    Alibabaou, Hossein Gharari
    [J]. CHIRALITY, 2014, 26 (05) : 260 - 267
  • [22] Simultaneous determination of six toxic alkaloids in human plasma and urine using capillary zone electrophoresis coupled to time-of-flight mass spectrometry
    Yu, Zhuhong
    Wu, Zhongping
    Gong, Feijun
    Wong, Rong
    Liang, Chen
    Zhang, Yurong
    Yu, Yunqiu
    [J]. JOURNAL OF SEPARATION SCIENCE, 2012, 35 (20) : 2773 - 2780
  • [23] Development and validation of a plasma assay for acyclovir using high-performance capillary electrophoresis with sample stacking
    Vo, HC
    Henning, PA
    Leung, DT
    Sacks, SL
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 772 (02): : 291 - 297
  • [24] Determination of phenprocoumon in plasma and urine using at-line solid-phase extraction capillary electrophoresis
    Veraart, JR
    Gooijer, C
    Lingeman, H
    Velthorst, NH
    Brinkman, UAT
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 17 (6-7) : 1161 - 1166
  • [25] Analysis of alendronate in human urine and plasma by magnetic solid-phase extraction and capillary electrophoresis with fluorescence detection
    Su, Siao-Wei
    Liao, Yu-Chen
    Whang, Chen-Wen
    [J]. JOURNAL OF SEPARATION SCIENCE, 2012, 35 (5-6) : 681 - 687
  • [26] Plasma and CSF pharmacokinetics of ganciclovir in nonhuman primates
    Serabe, BM
    Murry, DJ
    Dauser, R
    Nuchtern, J
    Durfee, J
    McGuffey, L
    Berg, S
    Blaney, SM
    [J]. CANCER CHEMOTHERAPY AND PHARMACOLOGY, 1999, 43 (05) : 415 - 418
  • [27] Determination of amitriptyline and nortriptyline in spiked plasma by capillary zone electrophoresis with β-cyclodextrin
    Wu, SM
    Wu, HL
    Ko, WK
    Chen, SH
    [J]. ANALYTICA CHIMICA ACTA, 2000, 413 (1-2) : 125 - 129
  • [28] A REVIEW ON ELECTROPHORESIS, CAPILLARY ELECTROPHORESIS AND HYPHENATIONS
    Gummadi, S.
    Kandula, V. N.
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICAL SCIENCES AND RESEARCH, 2020, 11 (12): : 6038 - 6056
  • [29] Rapid determination of short chain carnitines in human plasma by electrospray ionisation-ion trap mass spectrometry using capillary electrophoresis instrument as sampler
    Desiderio, Claudia
    Mancinelli, Angelo
    De Rossi, Antonella
    Rossetti, Diana Valeria
    Inzitari, Rosanna
    Messana, Irene
    Giardina, Bruno
    Castagnola, Massimo
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2007, 1150 (1-2) : 320 - 326
  • [30] Determination of Tricyclic Antidepressants in Human Breast Milk by Capillary Electrophoresis
    Acedo-Valenzuela, Maria-Isabel
    Mora-Diez, Nielene
    Galeano-Diaz, Teresa
    Silva-Rodriguez, Antonio
    [J]. ANALYTICAL SCIENCES, 2010, 26 (06) : 699 - 702