Molecular Detection, Characterisation and Serological Survey of Chicken Astrovirus from Broiler Flocks in Malaysia

被引：0
作者：
Raji, Abdullahi Abdullahi [1 ,2 ]
Ideris, Aini [1 ,3 ]
Bejo, Mohd Hair [4 ]
Omar, Abdul Rahman [1 ,4 ]
机构：
[1] Univ Putra Malaysia, Inst Biosci, Lab Vaccine & Biomol, UPM, Serdang 43400, Selangor, Malaysia
[2] Usmanu Danfodiyo Univ, Dept Vet Pathol, Fac Vet Med, City Campus Complex, Sokoto 840212, Sokoto State, Nigeria
[3] Univ Putra Malaysia, Dept Vet Clin Studies, UPM, Fac Vet Med, Serdang 43400, Selangor, Malaysia
[4] Univ Putra Malaysia, Dept Vet Pathol & Microbiol, UPM, Fac Vet Med, Serdang 43400, Selangor, Malaysia
来源：
PERTANIKA JOURNAL OF SCIENCE AND TECHNOLOGY | 2022年 / 30卷 / 02期
关键词：
Broiler breeder; broilers; chicken astrovirus; Malaysia; PCR; polymerase gene; LINKED-IMMUNOSORBENT-ASSAY; POLYMERASE-CHAIN-REACTION; AVIAN NEPHRITIS VIRUS; STUNTING SYNDROME; RT-PCR; IDENTIFICATION; TYPE-2;
D O I：
10.47836/pjst.30.2.43
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Astroviruses have been associated with enteric and extra-intestinal disorders in many animal species, including chickens. Here, we describe the detection and characterisation of chicken astrovirus (CAstV) in broilers and its seroprevalence in broiler breeder flocks. Based on PCR protocol, viral confirmation was carried out on clinical tissue samples from broiler chickens suffering from uneven growth and poor performance. The tissues were molecularly detected for CAstV with differential diagnostic testing against the Newcastle disease virus, infectious bronchitis virus, avian nephritis virus, avian rotavirus, fowl adenovirus and avian reovirus. Polymerase gene-based phylogenetic analyses of the twenty samples detected positive for CAstV indicate they belong to Group I and are related to strains from the US, UK, India and Poland. From these 20 samples, CastV could be isolated from 3 samples upon inoculation in 5-day-old specific-pathogen free (SPF) embryonated chicken eggs (ECE); virus-infected embryos showed dwarfing, haemorrhages, oedema and gelatinous lesions at harvest. The enzyme linked immunosorbent assay (ELISA) results revealed a high prevalence of antibodies against CAstV amongst the broiler breeder flocks tested. It is the first study that describes the detection and prevalence of CAstV in broiler chickens and broiler breeder flocks in Malaysia.
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页码：1641 / 1655
页数：15
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