Split Green Fluorescent Proteins: Scope, Limitations, and Outlook

被引：148

作者：

Romei, Matthew G. ^{[1
]}

Boxer, Steven G. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS, VOL 48 | 2019年 / 48卷

关键词：

split protein; green fluorescent protein; bimolecular fluorescence complementation; biosensor; photochemistry; FRAGMENT COMPLEMENTATION ASSAYS; IN-VIVO; LIVING CELLS; SIMULTANEOUS VISUALIZATION; OPTICAL CONTROL; GFP; RNA; SYSTEM; REPORTER; BINDING;

D O I：

10.1146/annurev-biophys-051013-022846

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.

引用

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页码：19 / 44

页数：26

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