H3K9 histone methyltransferase G9a-mediated transcriptional activation of p21

被引：29

作者：

Oh, Si-Taek ^{[1
]}

Kim, Kee-Beom ^{[1
]}

Chae, Yun-Cheol ^{[1
]}

Kang, Joo-Young ^{[1
]}

Hahn, Yoonsoo ^{[1
]}

Seo, Sang-Beom ^{[1
]}

机构：

[1] Chung Ang Univ, Coll Nat Sci, Dept Life Sci, Seoul 156756, South Korea

来源：

FEBS LETTERS | 2014年 / 588卷 / 05期

基金：

新加坡国家研究基金会;

关键词：

G9a; p21; Transcription; Apoptosis; Etoposide; G9A; CELLS; COACTIVATOR; PROTEIN; GLP; P53;

D O I：

10.1016/j.febslet.2014.01.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21, in p53-null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage-inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21-mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：685 / 691

页数：7

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