Unravelling the structural and mechanistic basis of CRISPR-Cas systems

被引：545

作者：

van der Oost, John ^{[1
]}

Westra, Edze R. ^{[1
,2
]}

Jackson, Ryan N. ^{[3
]}

Wiedenheft, Blake ^{[3
]}

机构：

[1] Wageningen Univ, Microbiol Lab, NL-6703 HB Wageningen, Netherlands

[2] Univ Exeter, Environm & Sustainabil Inst, Penryn TR10 9FE, Cornwall, England

[3] Montana State Univ, Dept Microbiol & Immunol, Bozeman, MT 59717 USA

来源：

NATURE REVIEWS MICROBIOLOGY | 2014年 / 12卷 / 07期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

ADAPTIVE IMMUNE-SYSTEMS; RNA RECOGNITION MOTIF; PROCESSES PRE-CRRNA; CRYSTAL-STRUCTURE; ANTIVIRAL DEFENSE; CMR2-CMR3; SUBCOMPLEX; INTERFERENCE COMPLEX; ACQUIRED-RESISTANCE; TARGET RECOGNITION; ESCHERICHIA-COLI;

D O I：

10.1038/nrmicro3279

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteria and archaea have evolved sophisticated adaptive immune systems, known as CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated proteins) systems, which target and inactivate invading viruses and plasmids. Immunity is acquired by integrating short fragments of foreign DNA into CRISPR loci, and following transcription and processing of these loci, the CRISPR RNAs (crRNAs) guide the Cas proteins to complementary invading nucleic acid, which results in target interference. In this Review, we summarize the recent structural and biochemical insights that have been gained for the three major types of CRISPR-Cas systems, which together provide a detailed molecular understanding of the unique and conserved mechanisms of RNA-guided adaptive immunity in bacteria and archaea.

引用

页码：479 / 492

页数：14

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