RAPD analysis of seed purity in a commercial hybrid cabbage (Brassica oleracea var. capitata) cultivar

被引：31
|
作者：
Crockett, PA
Bhalla, PL
Lee, CK
Singh, MB [1 ]
机构：
[1] Univ Melbourne, Inst Land & Food Resources, Plant Mol Biol & Biotechnol Res Lab, Parkville, Vic 3052, Australia
[2] Henderson Seed Grp Pty Ltd, Templestowe, Vic, Australia
来源：
GENOME | 2000年 / 43卷 / 02期
关键词：
Brassica; cabbage; RAPD; seed purity test; F-1-hybrid seed;
D O I：
10.1139/gen-43-2-317
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in a commercial F-1-hybrid cabbage (Brassica oleracea var. capitata) cultivar is demonstrated. Genomic DNA isolated from single ungerminated seed was found to be suitable for RAPD analysis. DNA from F-1-hybrid and its parental lines was subjected to RAPD screening with 36 random decamer arbitrary primers. A total of 241 scorable products were observed with 54 (22%) being polymorphic. The RAPD data showed that the parental lines of this commercial cabbage cultivar were not very closely related. Two primers were chosen for purity testing of the F-1-hybrid seeds. The sib (inbred seed; seed from self-pollination of parental lines) contamination results obtained by RAPD analysis were comparable to the commonly used grow-out trial and isozyme analysis, hence showing that RAPD analysis can be used for seed purity testing of commercial hybrid cabbage seeds.
引用
收藏
页码：317 / 321
页数：5
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