Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology

被引:25
作者
Biswas, C
Kell, B
Mant, C
Jewers, RJ
Cason, J
Muir, P
Raju, KS
Best, JM
机构
[1] UNITED MED & DENT SCH, DEPT GYNAECOL, LONDON SE1 7EH, ENGLAND
[2] UNITED MED & DENT SCH, DEPT VIROL, RICHARD DIMBLEBY LAB CANC VIROL, LONDON SE1 7EH, ENGLAND
关键词
D O I
10.1128/JCM.35.6.1560-1564.1997
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DIVA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P = 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.
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页码:1560 / 1564
页数:5
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