Direct detection of cysteine using functionalized BaTiO3 nanoparticles film based self-powered biosensor

被引：52

作者：

Selvarajan, Sophia ^{[1
]}

Alluri, Nagamalleswara Rao ^{[2
]}

Chandrasekhar, Arunkumar ^{[3
]}

Kim, Sang-Jae ^{[1
,3
,4
]}

机构：

[1] Jeju Natl Univ, Dept Adv Convergence Technol & Sci, Nanomat & Syst Lab, Jeju 690756, South Korea

[2] Jeju Natl Univ, Dept Mech Engn, Nanomat & Syst Lab, Jeju 690756, South Korea

[3] Jeju Natl Univ, Dept Mechatron Engn, Nanomat & Syst Lab, Jeju 690756, South Korea

[4] Jeju Natl Univ, Nano Mat & Syst Lab D130, Engn Bldg-4, Jeju Si 690756, Jeju Do, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2017年 / 91卷

基金：

新加坡国家研究基金会;

关键词：

NH2-functionalized BaTiO3 NPs; Agarose 3D matrix; Self-powered cysteine bio-sensor; I-V technique; Piezoelectric nanogenerator; Non-invasive (urine); SELECTIVE FLUORESCENT-PROBE; GLUCOSE SENSOR; HOMOCYSTEINE; PERFORMANCE; CYSTEINE/HOMOCYSTEINE; NANOGENERATOR; URINE; GATE;

D O I：

10.1016/j.bios.2016.12.006

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Simple, novel, and direct detection of clinically important biomolecules have continuous demand among scientific community as well as in market. Here, we report the first direct detection and facile fabrication of a cysteine-responsive, film-based, self-powered device. NH2 functionalized BaTiO3 nanoparticles (BT-NH2 NPs) suspended in a three-dimensional matrix of an agarose (Ag) film, were used for cysteine detection. BaTiO3 nanoparticles (BT NPs) semiconducting as well as piezoelectric properties were harnessed in this study. The changes in surface charge properties of the film with respect to cysteine concentrations were determined using a current voltage (I-V) technique. The current response increased with cysteine concentration (linear concentration range=10 M-1mM). Based on the properties of the composite (BT/Ag), we created a self-powered cysteine sensor in which the output voltage from a piezoelectric nanogenerator was used to drive the sensor. The potential drop across the sensor was measured as a function of cysteine concentrations. Real-time analysis of sensor performance was carried out on urine samples by non-invasive method. This novel sensor demonstrated good selectivity, linear concentration range and detection limit of 10 mu M; acceptable for routine analysis.

引用

页码：203 / 210

页数：8

共 35 条

[1] Flexible, Hybrid Piezoelectric Film (BaTi(1-x)ZrxO3)/PVDF Nanogenerator as a Self-Powered Fluid Velocity Sensor [J].