Construction of a compact spectrofluorometer/spectrophotometer system using a flexible liquid core waveguide

被引：42
作者：
Byrne, RH
Yao, WS
Kaltenbacher, E
Waterbury, RD
机构：
[1] Univ S Florida, Dept Marine Sci, St Petersburg, FL 33701 USA
[2] Ocean Opt Inc, Dunedin, FL 34698 USA
来源：
TALANTA | 2000年 / 50卷 / 06期
关键词：
spectrofluorometer; spectrophotometer; flexible liquid core waveguide; CCD array detection system;
D O I：
10.1016/S0039-9140(99)00239-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A liquid-core waveguide made of Teflon AF-2400 has been used to construct a simple, sensitive and robust instrument capable of performing fluorimetric and spectrophotometric analyses on aqueous solutions. The instrument. which uses a CCD array detection system, is unique in that high performance is achieved for both measurement techniques with minimal changes in instrument configuration. The fluorimetric detection limits for quinine sulfate and chlorophyll-a are 0.06 nanomolar and 0.03 nanomolar, respectively. Absorbance measurements using the same instrument demonstrate nanomolar detection capacities for hydrogen sulfide and subnanomolar detection limits for methylene blue. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：1307 / 1312
页数：6
相关论文
共 9 条
[1]   Low-loss liquid-core optical fiber for low-refractive-index liquids: fabrication, characterization, and application in Raman spectroscopy [J].
Altkorn, R ;
Koev, I ;
VanDuyne, RP ;
Litorja, M .
APPLIED OPTICS, 1997, 36 (34) :8992-8998
[2]   SPECTROPHOTOMETRIC DETERMINATION OF HYDROGEN SULFIDE IN NATURAL WATERS [J].
CLINE, JD .
LIMNOLOGY AND OCEANOGRAPHY, 1969, 14 (03) :454-&
[3]   Characterization of marine and terrestrial DOM in seawater using excitation emission matrix spectroscopy [J].
Coble, PG .
MARINE CHEMISTRY, 1996, 51 (04) :325-346
[4]   Luminescence detection with a liquid care waveguide [J].
Dasgupta, PK ;
Zhang, GF ;
Li, JZ ;
Boring, CB ;
Jambunathan, S ;
Al-Horr, R .
ANALYTICAL CHEMISTRY, 1999, 71 (07) :1400-1407
[5]   WAVE-GUIDE CAPILLARY-FLOW CELL FOR FLUOROMETRY [J].
FUJIWARA, K ;
SIMEONSSON, JB ;
SMITH, BW ;
WINEFORDNER, JD .
ANALYTICAL CHEMISTRY, 1988, 60 (10) :1065-1068
[6]  
SIERRA MMD, 1994, MAR CHEM, V47, P127
[7]   Long pathlength absorbance spectroscopy: trace analysis of Fe(II) using a 4.5m liquid core waveguide [J].
Waterbury, RD ;
Yao, WS ;
Byrne, RH .
ANALYTICA CHIMICA ACTA, 1997, 357 (1-2) :99-102
[8]   Determination of trace chromium(VI) and molybdenum(VI) in natural and bottled mineral waters using long pathlength absorbance spectroscopy (LPAS) [J].
Yao, WS ;
Byrne, RH .
TALANTA, 1999, 48 (02) :277-282
[9]   Determination of nanomolar concentrations of nitrite and nitrate in natural waters using long path length absorbance spectroscopy [J].
Yao, WS ;
Byrne, RH ;
Waterbury, RD .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1998, 32 (17) :2646-2649
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