Ultraviolet light and laser irradiation enhances the antibacterial activity of glucosamine-functionalized gold nanoparticles

被引：36

作者：

Govindaraju, Saravanan ^{[1
,3
]}

Ramasamy, Mohankandhasamy ^{[1
]}

Baskaran, Rengarajan ^{[2
]}

Ahn, Sang Jung ^{[3
,4
]}

Yun, Kyusik ^{[1
]}

机构：

[1] Gachon Univ, Dept Bionanotechnol, Gyeonggi Do 13120, South Korea

[2] Gachon Univ, Coll Pharm, Inchon, South Korea

[3] Univ Sci & Technol, Korea Res Inst Stand & Sci, Ctr Adv Instrumentat, Daejeon 34113, South Korea

[4] Univ Sci & Technol, Major Nano Sci, Daejeon 34113, South Korea

来源：

INTERNATIONAL JOURNAL OF NANOMEDICINE | 2015年 / 10卷

基金：

新加坡国家研究基金会;

关键词：

gold nanoparticles; glucosamine; light irradiation; antibacterial activity; bacterial morphology; DRUG-DELIVERY; BACTERIA; SILVER;

D O I：

10.2147/IJN.S88318

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Here we report a novel method for the synthesis of glucosamine-functionalized gold nanoparticles (GlcN-AuNPs) using biocompatible and biodegradable glucosamine for antibacterial activity. GlcN-AuNPs were prepared using different concentrations of glucosamine. The synthesized AuNPs were characterized for surface plasmon resonance, surface morphology, fluorescence spectroscopy, and antibacterial activity. The minimum inhibitory concentrations (MICs) of the AuNPs, GlcN-AuNPs, and GlcN-AuNPs when irradiated by ultraviolet light and laser were investigated and compared with the MIC of standard kanamycin using Escherichia coli by the microdilution method. Laser-irradiated GlcN-AuNPs exhibited significant bactericidal activity against E. coli. Flow cytometry and fluorescence microscopic analysis supported the cell death mechanism in the presence of GlcN-AuNP-treated bacteria. Further, morphological changes in E. coli after laser treatment were investigated using atomic force microscopy and transmission electron microscopy. The overall results of this study suggest that the prepared nanoparticles have potential as a potent antibacterial agent for the treatment of a wide range of disease-causing bacteria.

引用

页码：67 / 78

页数：12

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