Towards the elements of successful insect RNAi

被引:379
作者
Scott, Jeffrey G. [1 ]
Michel, Kristin [2 ]
Bartholomay, Lyric C. [3 ]
Siegfried, Blair D. [4 ]
Hunter, Wayne B. [5 ]
Smagghe, Guy [6 ]
Zhu, Kun Yan [7 ]
Douglas, Angela E. [1 ]
机构
[1] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA
[2] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA
[3] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA
[4] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA
[5] USDA ARS, Ft Pierce, FL 34945 USA
[6] Univ Ghent, Dept Crop Protect, B-9000 Ghent, Belgium
[7] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA
基金
美国国家科学基金会;
关键词
Antiviral therapy; dsRNA; Insect pest control; RNA interference; siRNA; Systemic RNAi; DOUBLE-STRANDED-RNA; RHODNIUS-PROLIXUS HEMIPTERA; ANOPHELES-GAMBIAE; SYSTEMIC RNAI; SINDBIS VIRUS; IN-VIVO; GENETIC INTERFERENCE; FUNCTIONAL GENOMICS; DENGUE VIRUSES; DSRNA;
D O I
10.1016/j.jinsphys.2013.08.014
中图分类号
Q96 [昆虫学];
学科分类号
摘要
RNA interference (RNAi), the sequence-specific suppression of gene expression, offers great opportunities for insect science, especially to analyze gene function, manage pest populations, and reduce disease pathogens. The accumulating body of literature on insect RNAi has revealed that the efficiency of RNAi varies between different species, the mode of RNAi delivery, and the genes being targeted. There is also variation in the duration of transcript suppression. At present, we have a limited capacity to predict the ideal experimental strategy for RNAi of a particular gene/insect because of our incomplete understanding of whether and how the RNAi signal is amplified and spread among insect cells. Consequently, development of the optimal RNAi protocols is a highly empirical process. This limitation can be relieved by systematic analysis of the molecular physiological basis of RNAi mechanisms in insects. An enhanced conceptual understanding of RNAi function in insects will facilitate the application of RNAi for dissection of gene function, and to fast-track the application of RNAi to both control pests and develop effective methods to protect beneficial insects and non-insect arthropods, particularly the honey bee (Apis mellifera) and cultured Pacific white shrimp (Litopenaeus vannamei) from viral and parasitic diseases. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1212 / 1221
页数:10
相关论文
共 122 条
  • [1] Sindbis virus-induced silencing of dengue viruses in mosquitoes
    Adelman, ZN
    Blair, CD
    Carlson, JO
    Beaty, BJ
    Olson, KE
    [J]. INSECT MOLECULAR BIOLOGY, 2001, 10 (03) : 265 - 273
  • [2] Saliva of Lygus lineolaris digests double stranded ribonucleic acids
    Allen, Margaret L.
    Walker, William B., III
    [J]. JOURNAL OF INSECT PHYSIOLOGY, 2012, 58 (03) : 391 - 396
  • [3] Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA
    Amdam, GV
    Simoes, ZLP
    Guidugli, KR
    Norberg, K
    Omholt, SW
    [J]. BMC BIOTECHNOLOGY, 2003, 3 (1)
  • [4] Laccase 2 is the phenoloxidase gene required for beetle cuticle tanning
    Arakane, Y
    Muthukrishnan, S
    Beeman, RW
    Kanost, MR
    Kramer, KJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (32) : 11337 - 11342
  • [5] Both UDP N-acetylglucosamine pyrophosphorylases of Tribolium castaneum are critical for molting, survival and fecundity
    Arakane, Yasuyuki
    Baguinon, Marilyn C.
    Jasrapuria, Sinu
    Chaudhari, Sujata
    Doyungan, Alison
    Kramer, Karl J.
    Muthukrishnan, Subbaratnam
    Beeman, Richard W.
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2011, 41 (01) : 42 - 50
  • [6] RNA interference of the salivary gland nitrophorin 2 in the triatomine bug Rhodnius prolixus (Hemiptera: Reduviidae) by dsRNA ingestion or injection
    Araujo, R. N.
    Santos, A.
    Pinto, F. S.
    Gontijo, N. F.
    Lehane, M. J.
    Pereira, M. H.
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2006, 36 (09) : 683 - 693
  • [7] Molecular characterization of a cDNA encoding extracellular dsRNase and its expression in the silkworm, Bombyx mori
    Arimatsu, Yuji
    Kotani, Eiji
    Sugimura, Yukio
    Furusawa, Toshiharu
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2007, 37 (02) : 176 - 183
  • [8] SID-I is implicated in systemic gene silencing in the honey bee
    Aronstein, Katherine
    Pankiw, Tanya
    Saldivar, Eduardo
    [J]. JOURNAL OF APICULTURAL RESEARCH, 2006, 45 (01) : 20 - 24
  • [9] MicroRNA functions in insects
    Asgari, Sassan
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2013, 43 (04) : 388 - 397
  • [10] Analysis of lipolysis underlying lactation in the tsetse fly, Glossina morsitans
    Attardo, Geoffrey M.
    Benoit, Joshua B.
    Michalkova, Veronika
    Yang, Guangxiao
    Roller, Ladislav
    Bohova, Jana
    Takac, Peter
    Aksoy, Serap
    [J]. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2012, 42 (05) : 360 - 370