Protein renaturation with simultaneous purification by protein folding liquid chromatography: recent developments

被引：12

作者：

Wang, Lili ^{[1
]}

Geng, Xindu ^{[1
]}

机构：

[1] NW Univ Xian, Key Lab Synthet & Nat Funct Mol Chem, Shaanxi Key Lab Modern Separat Sci, Minist Educ,Inst Modern Separat Sci, Xian 710069, Peoples R China

来源：

AMINO ACIDS | 2014年 / 46卷 / 01期

关键词：

Protein folding liquid chromatography (PFLC); Proteins; Inclusion bodies; Refolding with simultaneous purification; Mixed-mode liquid chromatography; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; COLONY-STIMULATING FACTOR; BED ADSORPTION CHROMATOGRAPHY; SIZE-EXCLUSION CHROMATOGRAPHY; MIXED-MODE CHROMATOGRAPHY; ION-EXCHANGE CHROMATOGRAPHY; INCLUSION-BODY PROTEIN; HIGH-LEVEL EXPRESSION; ESCHERICHIA-COLI; RHG-CSF;

D O I：

10.1007/s00726-013-1614-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein folding liquid chromatography (PFLC) is a powerful tool for protein refolding with simultaneous purification. We review its recent progress in liquid chromatography and molecular biology, primarily involving the validation of PFLC refolding of proteins containing multiple disulphide bonds, the application of mixed-mode chromatography, PFLC in molecular biology. Representative examples are described.

引用

页码：153 / 165

页数：13

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