MicroRNA-509-3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis

被引：24

作者：

Chen, Wei ^{[1
]}

Zeng, Wenshu ^{[2
]}

Li, Xiaodi ^{[2
]}

Xiong, Weiliang ^{[3
]}

Zhang, Mengdie ^{[2
]}

Huang, Yan ^{[2
]}

Zhou, Longshu ^{[1
]}

Jiang, Songshan ^{[2
]}

机构：

[1] Guangzhou Med Univ, Affiliated Hosp 2, Dept Gynecol, Guangzhou, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

[3] Gene Sci & Hlth Co, Shenzhen, Peoples R China

来源：

PHARMACOGENOMICS | 2016年 / 17卷 / 03期

基金：

中国国家自然科学基金;

关键词：

cell apoptosis; cell proliferation; microRNA-509-3p; ovarian cancer; X-linked inhibitor of apoptosis; HUMAN INHIBITOR; XIAP; EXPRESSION; RESISTANCE; PROTEINS; GENES; PHENOXODIOL; SIGNATURES; CARCINOMA; FAMILY;

D O I：

10.2217/pgs.15.166

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Aims: XIAP is upregulated in chemoresistant epithelial ovarian cancer (EOC). However, the molecular mechanism of this dysregulation remains unclear. Materials & methods: The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays. Results: miR-509-3p, a downregulated miRNA in chemoresistant EOC, can directly target the XIAP via its 3'UTR. Overexpression of miR-509-3p can not only downregulate the expression of XIAP in ovarian cancer cells but also inhibit the proliferation of EOC cells and increase their sensitivity to cisplatin-induced apoptosis. Conclusions: Our data suggest that restoring certain dysregulated miRNAs to their normal levels could increase the therapeutic effects of anticancer drugs.

引用

页码：187 / 197

页数：11

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