Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes

被引：10

作者：

Manabe, Yasuko ^{[1
]}

Ogino, Shinya ^{[1
]}

Ito, Miyuki ^{[1
]}

Furuichi, Yasuro ^{[1
]}

Takagi, Mayumi ^{[1
]}

Yamada, Mio ^{[1
]}

Goto-Inoue, Naoko ^{[1
,2
]}

Ono, Yusuke ^{[3
]}

Fujii, Nobuharu L. ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Human Hlth Sci, Dept Hlth Promot Sci, Hachioji, Tokyo 1920397, Japan

[2] Nihon Univ, Dept Marine Sci & Resources, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan

[3] Nagasaki Univ, Atom Bomb Dis Inst, Grad Sch Biomed Sci, Dept Stem Cell Biol, Nagasaki 8528523, Japan

来源：

ANALYTICAL BIOCHEMISTRY | 2016年 / 497卷

关键词：

Skeletal muscle; Primary cultured cells; Myotubes; Contraction; SKELETAL-MUSCLE; SATELLITE CELLS; SELF-RENEWAL; FIBER-TYPE; MYOSIN;

D O I：

10.1016/j.ab.2015.10.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：36 / 38

页数：3

共 50 条

[41] Measurement of Periodical Contraction of Cultured Muscle Tube with Laser
Okada, Masahide
Hashimoto, Shigehiro
Takase, Jun
Ohsuga, Mieko
Nakamura, Kana
Akazawa, Kenzo
Mochizuki, Shuichi
Kobayashi, Hiroyuki
Fujisato, Toshia
Kawai, Toshikazu
Uto, Sadahito
Tsujita, Katsuyoshi
Yamada, Eiji
Kondo, Hideo
WMSCI 2008: 12TH WORLD MULTI-CONFERENCE ON SYSTEMICS, CYBERNETICS AND INFORMATICS, VOL II, PROCEEDINGS, 2008, : 110 - 114
[42] Contraction of the cultured smooth muscle of porcine coronary artery
Bi, D
Nishimura, J
Hirano, K
Kanaide, H
JAPANESE JOURNAL OF PHARMACOLOGY, 2002, 88 : 253P - 253P
[43] Evaluation of the effect of smooth muscle cells on the quality of cultured meat in a model for cultured meat
Zheng, Yan-Yan
Zhu, Hao-Zhe
Wu, Zhong-Yuan
Song, Wen-Juan
Tang, Chang-Bo
Li, Chun-Bao
Ding, Shi-Jie
Zhou, Guang-Hong
FOOD RESEARCH INTERNATIONAL, 2021, 150
[44] BLOCK OF NICOTINIC ACETYLCHOLINE-ACTIVATED CHANNELS OF CULTURED MOUSE MYOTUBES BY ISOFLURANE
BUFLER, J
PICHLMEIER, R
SCHNECK, HJ
HUSSMAN, H
FRANKE, C
NEUROSCIENCE LETTERS, 1994, 168 (1-2) : 135 - 138
[45] Cationic lipids and polymers are able to enhance adenoviral infection of cultured mouse myotubes
Dodds, E
Piper, TA
Murphy, SJ
Dickson, G
JOURNAL OF NEUROCHEMISTRY, 1999, 72 (05) : 2105 - 2112
[46] Effects of FHC mutant β-cardiac myosin on myofibrillogenesis in cultured mouse myotubes.
Peckham, M
Miller, G
BIOPHYSICAL JOURNAL, 2000, 78 (01) : 393A - 393A
[47] Intrinsic ionic conductances mediate the spontaneous electrical activity of cultured mouse myotubes
Sciancalepore, M
Afzalov, R
Buzzin, V
Jurdana, M
Lorenzon, P
Ruzzier, F
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2005, 1720 (1-2): : 117 - 124
[48] Molecular model for muscle contraction
Holmes, K.C.
Acta Crystallographica, Section A: Foundations of Crystallography, 1998, 54 (1 pt 6): : 789 - 797
[49] Geometric model of muscle contraction
Hafner, I
Simunic, B
Valencic, V
Djordjevic, S
Stusek, P
Znidaric, G
IEEE REGION 8 EUROCON 2003, VOL B, PROCEEDINGS: COMPUTER AS A TOOL, 2003, : 206 - 208
[50] An in vitro human skeletal muscle model: coculture of myotubes, neuron-like cells, and the capillary network
Ertan, Ayse Burcu
Kenar, Halime
Beyzadeoglu, Tahsin
Kok, Fatma Nese
Kose, Gamze Torun
TURKISH JOURNAL OF BIOLOGY, 2017, 41 (03) : 514 - 525

← 1 2 3 4 5 →