Evaluation of an in vitro muscle contraction model in mouse primary cultured myotubes

被引：10
|
作者：
Manabe, Yasuko [1 ]
Ogino, Shinya [1 ]
Ito, Miyuki [1 ]
Furuichi, Yasuro [1 ]
Takagi, Mayumi [1 ]
Yamada, Mio [1 ]
Goto-Inoue, Naoko [1 ,2 ]
Ono, Yusuke [3 ]
Fujii, Nobuharu L. [1 ]
机构：
[1] Tokyo Metropolitan Univ, Grad Sch Human Hlth Sci, Dept Hlth Promot Sci, Hachioji, Tokyo 1920397, Japan
[2] Nihon Univ, Dept Marine Sci & Resources, Coll Bioresource Sci, Fujisawa, Kanagawa 2520880, Japan
[3] Nagasaki Univ, Atom Bomb Dis Inst, Grad Sch Biomed Sci, Dept Stem Cell Biol, Nagasaki 8528523, Japan
来源：
ANALYTICAL BIOCHEMISTRY | 2016年 / 497卷
关键词：
Skeletal muscle; Primary cultured cells; Myotubes; Contraction; SKELETAL-MUSCLE; SATELLITE CELLS; SELF-RENEWAL; FIBER-TYPE; MYOSIN;
D O I：
10.1016/j.ab.2015.10.010
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
To construct an in vitro contraction model with the primary cultured myotubes, we isolated satellite cells from the mouse extensor digitorum longus. Differentiated myotubes possessed a greater number of sarcomere assemblies and higher expression levels of myosin heavy chain, cytochrome c oxidase IV, and myoglobin than in C2C12 myotubes. In agreement with these results regarding the sarcomere assemblies and protein expressions, the primary myotubes showed higher contractile activity stimulated by the electric pulses than that in the C2C12 myotubes. These data suggest that mouse primary myotubes will be a valuable research tool as an in vitro muscle contraction model. (C) 2015 Elsevier Inc. All rights reserved.
引用
收藏
页码：36 / 38
页数：3
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