Effect of two fertilization medium on the in vitro development of Creole bovine embryos

被引：0

作者：

Goicochea Vargas, Jose ^{[1
]}

Rondon Jorge, Wilson ^{[1
]}

Acosta Pachorro, Fidel ^{[1
]}

Gomez Marin, Yusep ^{[1
]}

Montalvo Martin, Magaly ^{[1
]}

Salvatierra Alor, Max ^{[2
]}

Martel Falcon, Jessica ^{[1
]}

Ballarte Zevallos, Oscar ^{[1
]}

Diaz Zegarra, Julio ^{[1
]}

Ratto Fuster, Marcelo ^{[3
]}

机构：

[1] Univ Nacl Hermilio Valdizan UNHEVAL, Lab Cirugia & Biotecnol Reprod, Fac Med Vet & Zootecnia, Huanuco, Peru

[2] Univ Nacl Hermilio Valdizan UNHEVAL, Lab Biotecnol Mol, Huanuco, Peru

[3] Univ Austral Chile, Inst Ciencia Anim, Valdivia, Chile

来源：

REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU | 2021年 / 32卷 / 05期

关键词：

FERT-TALP; TCM-199; in vitro; embryos; native cattle; CULTURE; MATURATION; MORPHOLOGY; VIABILITY; KINETICS; OOCYTES; VIVO;

D O I：

10.15381/rivep.v32i5.18709

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.

引用

页数：9

共 27 条

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