TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells
被引:123
作者:
Paula-Silva, F. W. G.
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Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin Prevent & Social Dent, BR-14049 Ribeirao Preto, SP, BrazilUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
Paula-Silva, F. W. G.
[1
,2
]
Ghosh, A.
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Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USAUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
Ghosh, A.
[1
]
Silva, L. A. B.
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Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin Prevent & Social Dent, BR-14049 Ribeirao Preto, SP, BrazilUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
Silva, L. A. B.
[2
]
Kapila, Y. L.
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Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USAUniv Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
Kapila, Y. L.
[1
]
机构:
[1] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin Prevent & Social Dent, BR-14049 Ribeirao Preto, SP, Brazil
Dental pulp cells can differentiate toward an odontoblastic phenotype to produce reparative dentin beneath caries lesions. However, the mechanisms involved in pulp cell differentiation under pro-inflammatory stimuli have not been well-explored. Thus, we hypothesized that the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could be a mediator involved in dental pulp cell differentiation toward an odontoblastic phenotype. We observed that TNF-alpha-challenged pulp cells exhibited increased mineralization and early and increased expression of dentin phosphoprotein (DPP), dentin sialoprotein (DSP), dentin matrix protein-1, and osteocalcin during a phase of reduced matrix metalloproteinase (MMP) expression. We investigated whether these events were related and found that p38, a mitogen-activated protein kinase, differentially regulated MMP-1 and DSP/DPP expression and mediated mineralization upon TNF-alpha treatment. These findings indicate that TNF-alpha stimulates differentiation of dental pulp cells toward an odontoblastic phenotype via p38, while negatively regulating MMP-1 expression.