TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells

被引:123
作者
Paula-Silva, F. W. G. [1 ,2 ]
Ghosh, A. [1 ]
Silva, L. A. B. [2 ]
Kapila, Y. L. [1 ]
机构
[1] Univ Michigan, Sch Dent, Dept Periodont & Oral Med, Ann Arbor, MI 48109 USA
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin Prevent & Social Dent, BR-14049 Ribeirao Preto, SP, Brazil
关键词
dental pulp cells; tumor necrosis factor-alpha; dentin sialophosphoprotein; matrix metalloproteinase-1; p38; MAPK; NECROSIS-FACTOR-ALPHA; PERIODONTAL-LIGAMENT CELLS; STEM-CELLS; ALKALINE-PHOSPHATASE; GENE-EXPRESSION; MINERALIZATION MARKERS; DENTINOGENESIS; PROTEINS; KINASE; DIFFERENTIATION;
D O I
10.1177/0022034509334070
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Dental pulp cells can differentiate toward an odontoblastic phenotype to produce reparative dentin beneath caries lesions. However, the mechanisms involved in pulp cell differentiation under pro-inflammatory stimuli have not been well-explored. Thus, we hypothesized that the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could be a mediator involved in dental pulp cell differentiation toward an odontoblastic phenotype. We observed that TNF-alpha-challenged pulp cells exhibited increased mineralization and early and increased expression of dentin phosphoprotein (DPP), dentin sialoprotein (DSP), dentin matrix protein-1, and osteocalcin during a phase of reduced matrix metalloproteinase (MMP) expression. We investigated whether these events were related and found that p38, a mitogen-activated protein kinase, differentially regulated MMP-1 and DSP/DPP expression and mediated mineralization upon TNF-alpha treatment. These findings indicate that TNF-alpha stimulates differentiation of dental pulp cells toward an odontoblastic phenotype via p38, while negatively regulating MMP-1 expression.
引用
收藏
页码:339 / 344
页数:6
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