Antibacterial and antifungal activity of Flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam.

Aim of the study: The leaves and root of Toddalia asiatica (L.) Lam. (Rutaceae) are widely used as a folk medicine in India. Hexane. chloroform, ethyl acetate, methanol and water extracts of Toddalia asiatica leaves and isolated compound Flindersine were tested against bacteria and fungi. Materials and methods: Antibacterial and antifungal activities were tested against bacteria and fungi using disc-diffusion method and minimum inhibitory concentrations (MICs). The compound was confirmed using X-ray crystallography technique. Results: Antibacterial and antifungal activities were observed in ethyl acetate extract. One active principle Flindersine (2,6-dihydro-2,2-dimethyl-5H-pyrano [3,2-c] quinoline-5-one-9cl) was isolated from the ethyl acetate extract. The MIC values of the compound against bacteria Bacillus subtilis (31.25 mu g/ml), Staphylococcus aureus (62.5 mu g/ml), Staphylococcus epidermidis (62.5 mu g/ml), Enterococcus faecalis (31.25 mu g/ml), Pseudomonas aeruginosa (250 mu g/ml), Acinetobacter baumannii (125 mu g/ml) and fungi Trichophyton rubrum 57 (62.5 mu g/ml), Trichophyton mentagrophytes (62.5 mu g/ml), Trichophyton simii (62.5 mu g/ml), Epidermophyton floccosum (62.5 mu g/ml), Magnaporthe grisea (250 mu g/ml) and Candida albicans (250 mu g/ml) were determined. Conclusions: Ethyl acetate extract showed promising antibacterial and antifungal activity and isolated compound Flindersine showed moderate activity against bacteria and fungi. (C) 2009 Published by Elsevier Ireland Ltd.