Method performance and multi-laboratory assessment of a normal phase high pressure liquid chromatography-fluorescence detection method for the quantitation of flavanols and procyanidins in cocoa and chocolate containing samples

被引：99

作者：

Robbins, Rebecca J. ^{[1
]}

Leonczak, Jadwiga ^{[1
]}

Johnson, J. Christopher ^{[1
]}

Li, Julia ^{[1
]}

Kwik-Uribe, Catherine ^{[1
]}

Prior, Ronald L. ^{[2
]}

Gu, Liwei ^{[3
]}

机构：

[1] Mars Inc, Analyt & Appl Sci Grp, Hackettstown, NJ 07840 USA

[2] ARS, USDA, Arkansas Childrens Nutr Ctr, Little Rock, AR 72202 USA

[3] Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 24期

关键词：

Flavanols; Procyanidins; Cocoa; Multi-laboratory validation; Quantitative; HPLC; FLD; NIST; Chocolate; Diol; RICH COCOA; SPECTROMETRY ANALYSIS; ANTIOXIDANT CAPACITY; PLATELET ACTIVATION; THEOBROMA-CACAO; PROANTHOCYANIDINS; FOODS; QUANTIFICATION; BEVERAGES; HUMANS;

D O I：

10.1016/j.chroma.2009.04.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The quantitative parameters and method performance for a normal-phase HPLC separation of flavanols and procyanidins in chocolate and cocoa-containing food products were optimized and assessed. Single laboratory method performance was examined over three months using three separate secondary standards. RSD((r)) ranged from 1.9%, 4.5% to 9.0% for cocoa powder, liquor and chocolate samples containing 74.39, 15.47 and 1.87 mg/g flavanols and procyanidins, respectively. Accuracy was determined by comparison to the NIST Standard Reference Material 2384. Inter-lab assessment indicated that variability was quite low for seven different cocoa-containing samples, with a RSD((R)) of less than 10% for the range of samples analyzed. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4831 / 4840

页数：10

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