Effect of Porphyromonas gingivalis on epithelial cell MMP-9 type IV collagenase production

被引:25
作者
Fravalo, P [1 ]
Menard, C [1 ]
BonnaureMallet, M [1 ]
机构
[1] UNIV RENNES 1,UFR ODONTOL,EQUIPE BIOL BUCCALE EA1256,F-35000 RENNES,FRANCE
关键词
D O I
10.1128/IAI.64.12.4940-4945.1996
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Porphyromonas gingivalis is reportedly capable of stimulating the expression of host cell matrix metalloproteinases (MMP), contributing to tissue destruction However, the impact of this bacterium on specific molecules remains to be determined. In this study, we evaluate the effect of P. gingivalis on regulation of MMP-9 expression in human gingival epithelial cells (HGEC). Various inocula of P. gingivalis were added to cultures of HGEC. The effects of live bacteria, heat-killed bacteria, and outer membrane extract were analyzed. MMP-9 secretion by HGEC was evaluated by enzyme-linked immunosorbent assay. For inocula smaller than one bacterium per cell, the quantity of MMP-9 secreted by HGEC was increased in comparison to control conditions. For inocula from 2.5 to 250 bacteria per cell, an inhibition of MMP-9 secretion in a dose-response fashion was observed, with a maximum reduction (ranging from 80 to 95% in five experiments) at 50 bacteria per cell, Gelatin zymograms confirmed the decrease in MMP-9 secretion. A band of 83 kDa, corresponding to activated enzyme, mas present for inocula of 0.5 to 50 bacteria. Inhibition took place without any alteration of epithelial cell viability. Heat-killed bacteria and outer membrane extract also provoked proenzyme activation but did not inhibit MMP-9 secretion. These results demonstrate a direct effect of P. gingivalis on HGEC, suggesting a specific action on the collagen renewal process at the interface between the epithelium and connective tissue.
引用
收藏
页码:4940 / 4945
页数:6
相关论文
共 48 条
[11]  
FIVESTAYLOR PM, 1994, MOLECULAR PATHOGENESIS OF PERIODONTAL DISEASE, P57
[12]  
Genco R J, 1988, Adv Dent Res, V2, P245
[13]   HOST RESPONSES IN PERIODONTAL-DISEASES - CURRENT CONCEPTS [J].
GENCO, RJ .
JOURNAL OF PERIODONTOLOGY, 1992, 63 (04) :338-355
[14]   CHARACTERIZATION OF SODIUM DODECYL SULFATE-STABLE BACTEROIDES-GINGIVALIS PROTEASES BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
GRENIER, D ;
CHAO, G ;
MCBRIDE, BC .
INFECTION AND IMMUNITY, 1989, 57 (01) :95-99
[15]  
Grenier Daniel, 1993, P227
[16]   ELECTROPHORETIC ANALYSIS OF PLASMINOGEN ACTIVATORS IN POLYACRYLAMIDE GELS CONTAINING SODIUM DODECYL-SULFATE AND COPOLYMERIZED SUBSTRATES [J].
HEUSSEN, C ;
DOWDLE, EB .
ANALYTICAL BIOCHEMISTRY, 1980, 102 (01) :196-202
[17]  
HIBBS MS, 1985, J BIOL CHEM, V260, P2493
[18]  
HUARD A, IN PRESS IN VIGRO CE
[19]  
KJELDSEN L, 1992, EUR J HAEMATOL, V49, P180
[20]   NATURE OF COLLAGENOLYTIC ENZYME AND INHIBITOR ACTIVITIES IN CREVICULAR FLUID FROM HEALTHY AND INFLAMED PERIODONTAL TISSUES OF BEAGLE DOGS [J].
KRYSHTALSKYJ, E ;
SODEK, J .
JOURNAL OF PERIODONTAL RESEARCH, 1987, 22 (04) :264-269