Cytotoxicity and Hemocompatibility of a Family of Novel MeO-PEG-Poly (D,L-Lactic-co-glycolic acid)-PEG-OMe Triblock Copolymer Nanoparticles

被引：10

作者：

He, Lili ^{[1
,2
]}

Yang, Likai ^{[1
]}

Duan, Yourong ^{[3
]}

Deng, Li ^{[1
]}

Sun, Xun ^{[1
]}

Gu, Zhongwei ^{[4
]}

Zhang, Zhi-Rong ^{[1
]}

机构：

[1] Sichuan Univ, W China Sch Pharm, Key Lab Drug Targeting & Novel Drug Delivery Syst, Chengdu 610041, Peoples R China

[2] SW Univ Nationalities, Coll Chem & Environm Protect Engn, Chengdu 610041, Peoples R China

[3] Shanghai Jiao Tong Univ, Inst Canc, Shanghai Canc Inst, Shanghai 200032, Peoples R China

[4] Sichuan Univ, Natl Engn Res Ctr Biomat, Chengdu 610041, Peoples R China

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2009年 / 113卷 / 05期

基金：

美国国家科学基金会;

关键词：

copolymers; nanoparticles; composition-biocompatibility relationship; cytotoxicity; hemocompatibility; PLGA-MPEG NANOPARTICLES; CHANG LIVER-CELLS; IN-VITRO; BLOOD COMPATIBILITY; CHITOSAN NANOPARTICLES; DELIVERY; BIODISTRIBUTION; DNA;

D O I：

10.1002/app.30386

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

A family of newly synthesized monomethoxy (polyethylene glycol)-poly (D,L-lactic glycolic acid)monomethoxy(polyethylene glycol) (MeO-PEG-poly (D,L-lactic-co-glycolic acid)-PEG-OMe, PELGE) biodegradable polymers are candidates for intravenous nanoparticle drug, because of their merits of biocompatibility and blood compatibility, and their capability of escaping from the endothelium system (RES) and adsorbing proteins. In the current research, relationships between composition, cytotoxicity, and hemocompatibility of a series of blank PELGE nanoparticles were investigated. Cytotoxicity on Chang cell lines was investigated using the methyl thiazolyl tetrazolium (MTT) assay. Human and rabbit blood were used in studies of red blood cell hemolysis, whole blood clotting time, plasma recalcification profiles, and red blood cell form and appearance in whole blood. The results that the molecular weight of PEG used in the synthesis of polymers influenced their characteristics. Generally, as the molecular weight of PEG increased, increased cytotoxicity and hemocompatibility were observed. The RGR (relative growth rate) of PELGE nanoparticles synthesized with PEG 550 was above 70%, while that of PELGE nanoparticles synthesized with PEG 750 and PEG 2000 was in the range of 55-105% and 36-87%, respectively. For PELGE nanoparticles synthesized with PEG 550, most hemolysis values were in the range of 13%, while for PELGE nanoparticles synthesized with PEG 750 and PEG 2000 hemolysis values were 1-2%, and below 2%, respectively. None of the nanoparticles Caused changes in red blood cell form or appearance. Based on the results, 12 kinds of PELGE were chosen for further studies. (C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 113: 2933-2944, 2009

引用

页码：2933 / 2944

页数：12

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