The P170 expression system enhances hyaluronan molecular weight and production in metabolically-engineered Lactococcus lactis

被引：21

作者：

Chauhan, Ashish Singh ^{[1
]}

Badle, Sneh Sanjay ^{[1
]}

Ramachandran, K. B. ^{[1
]}

Jayaraman, Guhan ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Bhupat & Jyoti Mehta Sch Biosci, Madras 600036, Tamil Nadu, India

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2014年 / 90卷

关键词：

Hyaluronan; Biosynthesis; Recombinant DNA; Lactococcus lactis; Batch Processing; Fed-Batch Cultures; ACID PRODUCTION; STREPTOCOCCUS-ZOOEPIDEMICUS; BACILLUS-SUBTILIS; BIOSYNTHESIS; PROTEIN; CULTIVATION; INDUCTION; COLI;

D O I：

10.1016/j.bej.2014.05.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant Lactococcus lactis strains based on the P170 expression system were developed for hyaluronan (HA) production, by incorporating genes from the has operon of Streptococcus zooepidemicus and compared with nisin-inducible recombinant L. lactis strains containing the hasABC and hasABD constructs. It was found across all batch and fed-batch experimental studies that HA concentration and molecular weight (MW) were higher for the P170 expression systems than the corresponding NICE-based strains. The highest hyaluronan MW was obtained for all constructs in batch studies at 60 g/L initial glucose concentration, the highest being 2.94MDa for the P170 strains with hasABC construct (L. lactis APJ3). In fed-batch studies with constant feed rate, the L. lactis APJ3 gave better HA yield (0.03 g/g) than the NICE-based strain. A higher hyaluronan MW was obtained for all strains in pulse fed-batch compared to constant feed experiments, the highest being 2.52 MDa for L. lactis APJ3. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：73 / 78

页数：6

共 30 条

[1] High production of hyaluronic and lactic acids by Streptococcus zooepidemicus in fed-batch culture using commercial and marine peptones from fishing by-products [J].