Application of Lactobacillus johnsonii expressing phage endolysin for control of Clostridium perfringens

被引：40

作者：

Gervasi, T. ^{[1
,2
]}

Lo Curto, R. ^{[2
]}

Minniti, E. ^{[2
]}

Narbad, A. ^{[1
]}

Mayer, M. J. ^{[1
]}

机构：

[1] Inst Food Res, Gut Hlth & Food Safety Programme, Norwich NR4 7UA, Norfolk, England

[2] Univ Messina, Dipartimento Sci Ambiente Sicurezza Terr Alimenti, Messina, Italy

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2014年 / 59卷 / 04期

基金：

英国生物技术与生命科学研究理事会;

关键词：

antimicrobials; food safety; Lactobacillus; Clostridium perfringens; endolysin; LACTIC-ACID BACTERIA; NECROTIC ENTERITIS; BROILER-CHICKENS; PATHOGENESIS; POULTRY; TOXIN; GENES;

D O I：

10.1111/lam.12298

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Clostridium perfringens in frequently found in food and the environment and produces potent toxins that have a negative impact on both human and animal health and particularly on the poultry industry. Lactobacillus johnsonii FI9785, isolated from the chicken gastrointestinal tract, has been demonstrated to exclude Cl. perfringens in poultry. We have investigated the interaction of wild-type Lact. johnsonii FI9785 or an engineered strain expressing a cell wall-hydrolysing endolysin with Cl. perfringens in vitro, using a batch culture designed to simulate human gastrointestinal tract conditions. Co-culture experiments indicated that acid production by Lact. johnsonii is important in pathogen control. The co-culture of the endolysin-secreting Lact. johnsonii with Cl. perfringens showed that the engineered strain had the potential to control the pathogen, but the ability to reduce Cl. perfringens numbers was not consistent. Results obtained indicated that survival of high numbers of Lact. johnsonii will be essential for effective pathogen control.

引用

页码：355 / 361

页数：7

共 27 条

[1] A phase I trial with Transgenic bacteria expressing interleukin-10 in Crohn's disease [J].