Super-resolution Confocal Live Imaging Microscopy (SCLIM) Cutting-edge Technology in Cell Biology

被引:0
作者
Nakano, Akihiko [1 ]
机构
[1] RIKEN Ctr Adv Photon, Live Cell Mol Imaging Res Team, Wako, Saitama 3510198, Japan
来源
2013 35TH ANNUAL INTERNATIONAL CONFERENCE OF THE IEEE ENGINEERING IN MEDICINE AND BIOLOGY SOCIETY (EMBC) | 2013年
关键词
ER EXIT SITES; GOLGI-APPARATUS;
D O I
暂无
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Super-resolution confocal live imaging microscopy (SCLIM) we developed provides amazingly high-speed live cell imaging at high space resolution. With this technology we are now able to observe details of membrane traffic events, including behaviors of small vesicles, cisternal maturation of the Golgi apparatus, and membrane segregation within a compartment.
引用
收藏
页码:133 / 135
页数:3
相关论文
共 7 条
[1]   Membrane Traffic Within the Golgi Apparatus [J].
Glick, Benjamin S. ;
Nakano, Akihiko .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 2009, 25 :113-132
[2]   cis-Golgi proteins accumulate near the ER exit sites and act as the scaffold for Golgi regeneration after brefeldin A treatment in tobacco BY-2 cells [J].
Ito, Yoko ;
Uemura, Tomohiro ;
Shoda, Keiko ;
Fujimoto, Masaru ;
Ueda, Takashi ;
Nakano, Akihiko .
MOLECULAR BIOLOGY OF THE CELL, 2012, 23 (16) :3203-3214
[3]   Live imaging of yeast Golgi cisternal maturation [J].
Matsuura-Tokita, Kumi ;
Takeuchi, Masaki ;
Ichihara, Akira ;
Mikuriya, Kenta ;
Nakano, Akihiko .
NATURE, 2006, 441 (7096) :1007-1010
[4]   Spinning-disk confocal microscopy - A cutting-edge tool for imaging of membrane traffic [J].
Nakano, A .
CELL STRUCTURE AND FUNCTION, 2002, 27 (05) :349-355
[5]   Passage through the Golgi [J].
Nakano, Akihiko ;
Luini, Alberto .
CURRENT OPINION IN CELL BIOLOGY, 2010, 22 (04) :471-478
[6]   High-curvature domains of the ER are important for the organization of ER exit sites in Saccharomyces cerevisiae [J].
Okamoto, Michiyo ;
Kurokawa, Kazuo ;
Matsuura-Tokita, Kumi ;
Saito, Chieko ;
Hirata, Ryogo ;
Nakano, Akihiko .
JOURNAL OF CELL SCIENCE, 2012, 125 (14) :3412-3420
[7]   The Yeast Golgi Apparatus [J].
Suda, Yasuyuki ;
Nakano, Akihiko .
TRAFFIC, 2012, 13 (04) :505-510