Regulation of the yeast phospholipid hydroperoxide glutathione peroxidase GPX2 by oxidative stress is mediated by Yap1 and Skn7

被引：42
作者：
Tsuzi, D [1 ]
Maeta, K [1 ]
Takatsume, Y [1 ]
Izawa, S [1 ]
Inoue, Y [1 ]
机构：
[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
来源：
FEBS LETTERS | 2004年 / 565卷 / 1-3期
关键词：
glutathione peroxidase; Yap1; Skn7; yeast; oxidative stress;
D O I：
10.1016/j.febslet.2004.03.091
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The GPX2 gene encodes a homologue of phospholipid hydroperoxide glutathione peroxidase in Saccharomyces cerevisiae. The GPX2 promoter contains three elements the sequence of which is completely consistent with the optimal sequence for the Yap1 response element (YRE). Here, we identify the intrinsic YRE that functions in the oxidative stress response of GPX2. In addition, we discovered a cis-acting element (5'-GGCCGGC-3') within the GPX2 promoter proximal to the functional YRE that is necessary for H2O2-induced expression of GPX2. We present evidence showing that Skn7 is necessary for the oxidative stress response of GPX2 and is able to bind to this sequence. We determine the optimal sequence for Skn7 to regulate GPX2 under conditions of oxidative stress to be 5'-GGC(C/T)GGC-3', and we designate this sequence the oxidative stress-responsive Skn7 response element. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：148 / 154
页数：7
相关论文
共 23 条
  • [1] AP1-mediated multidrug resistance in Saccharomyces cerevisiae requires FLR1 encoding a transporter of the major facilitator superfamily
    Alarco, AM
    Balan, I
    Talibi, D
    Mainville, N
    Raymond, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (31) : 19304 - 19313
  • [2] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
  • [3] SKN7, A YEAST MULTICOPY SUPPRESSOR OF A MUTATION AFFECTING CELL-WALL BETA-GLUCAN ASSEMBLY, ENCODES A PRODUCT WITH DOMAINS HOMOLOGOUS TO PROKARYOTIC 2-COMPONENT REGULATORS AND TO HEAT-SHOCK TRANSCRIPTION FACTORS
    BROWN, JL
    NORTH, S
    BUSSEY, H
    [J]. JOURNAL OF BACTERIOLOGY, 1993, 175 (21) : 6908 - 6915
  • [4] A thiol peroxidase is an H2O2 receptor and redox-transducer in gene activation
    Delaunay, A
    Pflieger, D
    Barrault, MB
    Vinh, J
    Toledano, MB
    [J]. CELL, 2002, 111 (04) : 471 - 481
  • [5] Yap, a novel family of eight bZIP proteins in Saccharomyces cerevisiae with distinct biological functions
    Fernandes, L
    RodriguesPousada, C
    Struhl, K
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (12) : 6982 - 6993
  • [6] Analysis of a meiosis-specific URS1 site: Sequence requirements and involvement of replication protein A
    GailusDurner, V
    Chintamaneni, C
    Wilson, R
    Brill, SJ
    Vershon, AK
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (07) : 3536 - 3546
  • [7] YAP1P, A YEAST TRANSCRIPTIONAL ACTIVATOR THAT MEDIATES MULTIDRUG-RESISTANCE, REGULATES THE METABOLIC STRESS-RESPONSE
    GOUNALAKI, N
    THIREOS, G
    [J]. EMBO JOURNAL, 1994, 13 (17) : 4036 - 4041
  • [8] Genetic analysis of glutathione peroxidase in oxidative stress response of Saccharomyces cerevisiae
    Inoue, Y
    Matsuda, T
    Sugiyama, K
    Izawa, S
    Kimura, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (38) : 27002 - 27009
  • [9] Expression of the glyoxalase I gene of Saccharomyces cerevisiae is regulated by high osmolarity glycerol mitogen-activated protein kinase pathway in osmotic stress response
    Inoue, Y
    Tsujimoto, Y
    Kimura, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (05) : 2977 - 2983
  • [10] The essential protein Fap7 is involved in the oxidative stress response of Saccharomyces cerevisiae
    Juhnke, H
    Charizanis, C
    Latifi, F
    Krems, B
    Entian, KD
    [J]. MOLECULAR MICROBIOLOGY, 2000, 35 (04) : 936 - 948
123