Regulation of the yeast phospholipid hydroperoxide glutathione peroxidase GPX2 by oxidative stress is mediated by Yap1 and Skn7
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Tsuzi, D
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Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, JapanKyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
Tsuzi, D
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Maeta, K
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Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, JapanKyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
Maeta, K
[1
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Takatsume, Y
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Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, JapanKyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
Takatsume, Y
[1
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Izawa, S
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Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, JapanKyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
Izawa, S
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Inoue, Y
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[1] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Mol Microbiol Lab, Kyoto 6110011, Japan
The GPX2 gene encodes a homologue of phospholipid hydroperoxide glutathione peroxidase in Saccharomyces cerevisiae. The GPX2 promoter contains three elements the sequence of which is completely consistent with the optimal sequence for the Yap1 response element (YRE). Here, we identify the intrinsic YRE that functions in the oxidative stress response of GPX2. In addition, we discovered a cis-acting element (5'-GGCCGGC-3') within the GPX2 promoter proximal to the functional YRE that is necessary for H2O2-induced expression of GPX2. We present evidence showing that Skn7 is necessary for the oxidative stress response of GPX2 and is able to bind to this sequence. We determine the optimal sequence for Skn7 to regulate GPX2 under conditions of oxidative stress to be 5'-GGC(C/T)GGC-3', and we designate this sequence the oxidative stress-responsive Skn7 response element. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.