cDNA cloning and in situ hybridization of Δ11-desaturase, a key enzyme of pheromone biosynthesis in Ostrinia scapulalis (Lepidoptera: Crambidae)

被引：11

作者：

Fukuzawa, Mai ^{[1
]}

Fu, Xiaoyan ^{[1
]}

Tatsuki, Sadahiro ^{[1
]}

Ishikawa, Yukio ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Agr & Life Sci, Lab Appl Entomol, Tokyo 113857, Japan

来源：

JOURNAL OF INSECT PHYSIOLOGY | 2006年 / 52卷 / 05期

基金：

日本学术振兴会;

关键词：

in situ hybridization; Delta; 11-desaturase; pheromone biosynthesis; Ostrinia; fatty acid pheromone analog;

D O I：

10.1016/j.jinsphys.2005.11.004

中图分类号：

Q96 [昆虫学];

学科分类号：

摘要：

Female sex pheromones are considered to be produced in a "pheromone gland" located in the terminal abdominal segments (8th-10th, TAS) of a moth; however, in many moth species, the cells that produce pheromones have not actually been specified. We investigated cells in the TAS that synthesize pheromones in the adzuki bean borer Ostrinia scapulalis, by locating pheromones and their precursors, and mRNA for Delta 11-desaturase, a key enzyme in pheromone biosynthesis. We demonstrated that the pheromone components, (E)-11-and (Z)-11-tetradecenyl acetates, and their fatty acyl precursors were specifically contained in the dorsal part of the TAS. A cDNA (OscaZ/E11) that encodes a Delta 11-desaturase was cloned from the TAS. RT-PCR and in situ hybridization unequivocally showed that OscaZ/E11 is specifically expressed in the modified epidermal cells located at the dorsal end of the 8th-9th intersegmental membrane. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：430 / 435

页数：6

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