Restriction of 16S-23S intergenic rDNA for diversity evaluation of Azospirillum amazonense isolated from different Brachiaria spp.

被引:3
作者
dos Reis Junior, Fabio Bueno
Reis, Veronica Massena
Teixeira, Katia Regina dos Santos
机构
[1] Embrapa Cerrados, BR-73301970 Planaltina, DF, Brazil
[2] Embrapa Agrobiol, BR-23851970 Seropedica, RJ, Brazil
关键词
biological nitrogen fixation; diazotrophic bacteria; microbial ecology; pastures;
D O I
10.1590/S0100-204X2006000300009
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
The aim of this work was to study the intra-specific diversity of Azospirillum amazonense isolates and to establish possible influences of different Brachiaria spp. and edaphoclimatic conditions. The characterization of the diversity among the isolates of A. amazonense studied was conducted using restriction analysis of the 16S-23S rDNA intergenic spacer region. The evaluated strains were separated in two groups, defined at 56% of similarity. Brachiaria spp. showed effects on strain diversity. Most part of the isolates from B. decumbens and B. brizantha are inserted in the first group, while B. humidicola isolates concentrate in the second group.
引用
收藏
页码:431 / 438
页数:8
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