Caspian White Fish (Rutilus frisii kutum) as a host for Spring Viraemia of Carp Virus

Rutilus frisii kutum is a fish of the Cyprinidae Family which is native in Caspian Sea and commercially cultured in Iran. This study was conducted to investigate susceptibility of Caspian White Fish to Spring Viraemia of Carp Virus (SVCV) infection and to evaluate influence of different challenge routes on virulence of the virus. Fingerlings were infected by immersion, intra-peritoneal (i.p.) injection, cohabitation and orally. Dead and surviving fish were collected for histological examination as well as for virus re-isolation by cell culture, Reverse Transcriptase Polymerization Chain Reaction (RT-PCR) and Indirect Fluorescent Antibody Test (IFAT) analysis. The results indicated that immersion was the best infectious route of transmission with the highest mortality, whereas oral transmission showed the lowest mortality. The virus was also re-isolated from dead fish and identified by IFAT. In addition, histopathological changes including branchial, hepatic and splenic necrosis as well as glomerulonephritis and necrosis in kidney were observed in diseased fish tissues but not in the survivors. RT-PCR on samples obtained from surviving fish tissues detected viral genome in the fish surviving from immersion, i.p. injection and cohabitation challenges but not in the fish infected orally. In conclusion, Caspian White Fish are susceptible to infection by SVCV and virulence of the virus could be influenced by route of transmission. In addition, SVCV could persist in surviving fish, which may serve as reservoirs of the virus, transmitting infection to healthy fish population. (C) 2014 Elsevier B.V. All rights reserved.