Functional analysis of the genes of yeast chromosome V by genetic footprinting

被引：190
作者：
Smith, V
Chou, KN
Lashkari, D
Botstein, D
Brown, PO
机构：
[1] STANFORD UNIV,SCH MED,HOWARD HUGHES MED INST,STANFORD,CA 94305
[2] STANFORD UNIV,SCH MED,DEPT BIOCHEM,STANFORD,CA 94305
[3] STANFORD UNIV,SCH MED,DEPT GENET,STANFORD,CA 94305
来源：
SCIENCE | 1996年 / 274卷 / 5295期
关键词：
D O I：
10.1126/science.274.5295.2069
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.
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页码：2069 / 2074
页数：6
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