AFM imaging of functionalized carbon nanotubes on biological membranes

被引：40

作者：

Lamprecht, C. ^{[1
]}

Liashkovich, I. ^{[2
]}

Neves, V. ^{[3
]}

Danzberger, J. ^{[1
]}

Heister, E. ^{[3
]}

Rangl, M. ^{[1
]}

Coley, H. M. ^{[3
]}

McFadden, J. ^{[3
]}

Flahaut, E. ^{[4
,5
]}

Gruber, H. J. ^{[1
]}

Hinterdorfer, P. ^{[1
]}

Kienberger, F. ^{[1
,6
]}

Ebner, A. ^{[1
]}

机构：

[1] Johannes Kepler Univ Linz, Inst Biophys, A-4040 Linz, Austria

[2] Westfael Wilhelms Univ, Inst Physiol 2, D-48149 Munster, Germany

[3] Univ Surrey, Fac Hlth & Med Sci, Guildford GU2 7XH, Surrey, England

[4] CNRS, Inst Carnot Cirimat, F-31062 Toulouse, France

[5] Univ Toulouse, UPS, INP, Inst Carnot Cirimat, F-31062 Toulouse 9, France

[6] Agilent Labs Linz, A-4040 Linz, Austria

来源：

NANOTECHNOLOGY | 2009年 / 20卷 / 43期

关键词：

ATOMIC-FORCE MICROSCOPE; DRUG-DELIVERY; TRANSPORTERS; TRANSLOCATION; PROTEINS; RNA; DNA;

D O I：

10.1088/0957-4484/20/43/434001

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Multifunctional carbon nanotubes are promising for biomedical applications as their nano-size, together with their physical stability, gives access into the cell and various cellular compartments including the nucleus. However, the direct and label-free detection of carbon nanotube uptake into cells is a challenging task. The atomic force microscope (AFM) is capable of resolving details of cellular surfaces at the nanometer scale and thus allows following of the docking of carbon nanotubes to biological membranes. Here we present topographical AFM images of non-covalently functionalized single walled (SWNT) and double walled carbon nanotubes (DWNT) immobilized on different biological membranes, such as plasma membranes and nuclear envelopes, as well as on a monolayer of avidin molecules. We were able to visualize DWNT on the nuclear membrane while at the same time resolving individual nuclear pore complexes. Furthermore, we succeeded in localizing individual SWNT at the border of incubated cells and in identifying bundles of DWNT on cell surfaces by AFM imaging.

引用

页数：7

共 31 条

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