Purification of PIA and rSesC as Putative Vaccine Candidates Against Staphylococcus aureus

被引：0

作者：

Bahonar, Sara ^{[1
,2
]}

Ghazvinian, Maryam ^{[1
]}

Haghshenas, Mohamad Reza ^{[1
]}

Goli, Hamid Reza ^{[1
]}

Mirzaei, Bahman ^{[1
,2
]}

机构：

[1] Mazandaran Univ Med Sci, Dept Med Microbiol & Virol, Fac Med, Sari, Iran

[2] Mazandaran Univ Med Sci, Mol & Cell Biol Res Ctr, Fac Med, Sari, Iran

来源：

REPORTS OF BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2019年 / 8卷 / 02期

关键词：

Biofilm formation; Polysaccharide Intracellular Adhesin; Staphylococcus aureus; BIOFILM; EPIDERMIDIS; INFECTIONS; QUANTITATION;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Staphylococcus aureus is predominant at sites of biomaterial-associated infection (BAI) and frequently infects hospitalized individuals. Methods: The polysaccharide intercellular adhesin (PIA) and S. epidermidis rSesC protein, major macromolecules in biofilm formation, were purified under native conditions and cloned and expressed in a prokaryotic host. Results: Purification of the macromolecules was confirmed by FTIR and Western blotting. Conclusions: The S. epidermidis SesC protein and PIA were successfully purified. Both are considered as vaccine candidates.

引用

页码：161 / 167

页数：7

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