Enantioseparation of benproperine phosphate by capillary electrophoresis and its application

A new method for the chiral separation of benproperine phosphate enantiomers is reported. The enantioseparation of benproperine phosphate was obtained by capillary electrophoresis using hydroxyproply-gamma-cyclodextrin (HP-gamma-CD) as chiral selector. When 100 mmol/L Tris-H-3 PO4 buffer at pH 3.0 containing 25 mmol/L HP-gamma-CD was chosen as a running electrolyte, the benproperine phosphate enantiomers was resolved completely within 25 min and the resolution reached up to 2.03. Coupled with ultraviolet detection, an excellent linear range from 0.5 mg/L to 10 mg/L for the detemination of benproperine phosphate enantiomers was exhibited. The enantioseparation. method has been applied for the determination of benproperine phosphate enantiomer spiked in serum samples with satisfactory results. A field-amplified injection procedure was also investigated as a simple and effective way to enhance the sensitivity for the determination, this procedure can improve the detection sensitivity of benproperine phosphate enantiomers up to 23 folds.