Optimization of a capillary electrophoresis-electrospray mass spectrometry method for the quantitation of the 20 natural amino acids in childrens blood

被引：35

作者：

Martin-Girardeau, A ^{[1
]}

Renou-Gonnord, MF ^{[1
]}

机构：

[1] Ecole Polytech, CNRS, UMR 7651, Lab Mecanismes React, F-91128 Palaiseau, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2000年 / 742卷 / 01期

关键词：

amino acids;

D O I：

10.1016/S0378-4347(00)00157-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper describes useful information on the capillary electrophoresis-electrospray ionization mass spectroscopy (CE-ESIMS) interfacing for the analysis of amino acids (AAs) in standard mixtures and in child plasma blood serum. The developed procedure allows quantitation of the 20 natural AAs, in a single run, without any derivatization. Limits of detection as low as 3-20 mu mol/l (5-30 pg injected) per analyte were obtained with an efficiency of about 100 000 plates and a peak area relative standard deviation below 4%. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：163 / 171

页数：9

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