A novel murine antibody and an open sandwich immunoassay for the detection of clenbuterol

被引：21

作者：

Cong, Yang ^{[1
]}

Dong, Hang ^{[2
]}

Wei, Xiaoyuan ^{[3
]}

Zhang, Liqian ^{[1
]}

Bai, Jingkun ^{[1
]}

Wu, Jingliang ^{[1
]}

Huang, Johnny X. ^{[1
]}

Gao, Zhiqin ^{[1
]}

Ueda, Hiroshi ^{[4
,5
]}

Dong, Jinhua ^{[1
,5
]}

机构：

[1] Weifang Med Univ, Sch Biosci & Technol, Weifang Key Lab Antibody Med, Key Lab Biol Med Shandong Univ, Weifang 261053, Peoples R China

[2] Peking Univ, Sch Basic Med Sci, Beijing 100191, Peoples R China

[3] Tsinghua Univ, Sch Life Sci, Beijing 100000, Peoples R China

[4] Tokyo Inst Technol, Inst Innovat Res, Lab Chem & Life Sci, Yokohama, Kanagawa 2268503, Japan

[5] Tokyo Inst Technol, Inst Innovat Res, World Res Hub Initiat, Yokohama, Kanagawa 2268503, Japan

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2019年 / 182卷

基金：

中国国家自然科学基金; 日本学术振兴会;

关键词：

Clenbuterol; Monoclonal antibody; Immunoassay; Open sandwich ELISA; QUANTITATION;

D O I：

10.1016/j.ecoenv.2019.109473

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Clenbuterol (CLEN) is a sympathomimetic amine used as a decongestant and bronchodilator while treating breathing disorders. It is also used in food-producing animals as it improves the rate of red meat production. However, it is prohibited in many countries nowadays due to human health and safety concerns. Unfortunately, the illegal use of CLEN is still rampant. Thus, monitoring it in food and livestock is important. Here, we report a novel murine antibody and an open sandwich enzyme linked immunosorbent assay (OS-ELISA) to detect CLEN based on antigen-antibody reactions. The genes of antibody variable regions in mice immunized with CLEN conjugated with bovine serum albumin were cloned into a phagemid (pDong1/Fab) to construct a phage-display antibody library, from which a novel antibody, A12, was selected. Then, an OS-ELISA was developed to detect CLEN using separated variable regions of the A12 antibody. The limit of detection of the assay was found to be 8 ng/mL, which was useful for monitoring CLEN usage.

引用

页数：6

共 27 条

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