Rapid detection of natriuretic peptides by a microfluidic LabChip analyzer with DNA aptamers: Application of natriuretic peptide detection

被引：18

作者：

Lin, Ming-Cheng ^{[2
,3
]}

Nawarak, Jiraporn ^{[1
]}

Chen, Tai-Yuan ^{[4
]}

Tsai, Hsien-Yu ^{[1
,5
]}

Hsieh, Jung-Feng ^{[6
]}

Sinchaikul, Supachok ^{[1
]}

Chen, Shui-Tein ^{[1
,5
]}

机构：

[1] Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan

[2] Chung Shan Med Univ Hosp, Div Cardiol, Dept Internal Med, Taichung 40201, Taiwan

[3] Chung Shan Med Univ Hosp, Sch Med, Taichung 40201, Taiwan

[4] Chang Gung Mem Hosp, Dept Med Res, Clin Genom & Prote Core Lab, Kaohsiung 833, Taiwan

[5] Natl Taiwan Univ, Coll Life Sci, Inst Biochem Sci, Taipei 10617, Taiwan

[6] Fu Jen Catholic Univ, Dept Food Sci, Taipei 242, Taiwan

来源：

BIOMICROFLUIDICS | 2009年 / 3卷 / 03期

关键词：

IN-VITRO SELECTION; NUCLEIC-ACID SELECTION; PEGAPTANIB; MOLECULES; BIND; AGE;

D O I：

10.1063/1.3194283

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid detection of brain natriuretic peptide (BNP) concentration can be used for the diagnosis of acute heart failure and for the evaluation of the effectiveness of a clinical therapy. We used the systematic evolution of ligands by exponential enrichment method to develop DNA aptamers for BNP whose sequences were determined by cloning method and consensus sequence analysis. A total of eight conserved sequences was identified. By combining the fluorescent-labeled aptamers with fast protein lab-on-chip analysis, we could achieve quantification of BNP concentrations with high speed, sensitivity, and specificity. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3194283]

引用

页数：8

共 39 条

[1] Nucleic acid selection as a tool for drug discovery [J].